BSL-3实验室医疗废弃物专用包装袋的封口方式及层数对高压灭菌效果的影响

Impact of sealing method and layer number of autoclave bag on autoclave sterilization effect in the BSL-3 laboratory

目的对感染性废物的包装给出一个合理的包装层数和封口方式,在指定的高压灭菌程序下,达到更好的高压灭菌效果。方法在指定的高压程序P4、P9下,针对医疗废弃物专用包装袋采用不同的包装层数和封口方式(包装层数分别为单层、双层、三层;封口方式分别是轻微折叠、高压胶带、尼龙扎带封口);通过用生物指示剂对内部感染性废物的上部、中部、底部进行检测,评价不同组合方式下的高压灭菌效果。结果1)在P4高压程序下,三层医疗废弃物专用包装袋包装封口方式按轻微折叠、高压胶带、尼龙扎带封口,内部感染性废物上部、中部、下部的生物指示剂的阴性率分别为上部是100%、0%、0%,中部是45%、0%、0%,底部都是0%。二层医疗废弃物专用包装袋包装封口方式按轻微折叠、高压胶带、尼龙扎带封口,内部感染性废物上部、中部、底部的生物指示剂的阴性率分别为上部是100%、85%、0%,中部是67%、20%、0%,底部是10%、0%、0%。一层医疗废弃物专用包装袋包装封口方式按轻微折叠、高压胶带、尼龙扎带封口,内部感染性废物上部、中部、底部的生物指示剂的阴性率分别为上部是100%、100%、0%,中部是70%、45%、0%,底部是12.5%、0%、0%。(2)在P9高压程序下,三层医疗废弃物专用包装袋包装封口方式按轻微折叠、高压胶带、尼龙扎带封口,内部感染性废物上部、中部、底部的生物指示剂的阴性率分别为上部均是100%,中部是100%、100%、70%,底部是100%、100%、30%。二层医疗废弃物专用包装袋包装封口方式按轻微折叠、高压胶带、尼龙扎带封口,内部感染性废物上部、中部、底部的生物指示剂的阴性率分别为上部均是100%,中部均是100%,底部是100%、100%、60%。一层医疗废弃物专用包装袋包装封口方式按轻微折叠、高压胶带、尼龙扎带封口,内部感染性废物上部、中部、底部的生物指示剂的阴性率分别均为100%。结论高压感染性废物时,采用单层医疗废弃物专用包装袋,不封口的方式进行高压,高压灭菌效果最好。必须封口时,应尽量避免将医疗废弃物专用包装袋的袋口封的过紧,以免由于蒸汽不能完全渗透,导致感染性废物达不到完全灭菌。

Objective To evaluate,the method of different packing layer and sealing mode for the packaging of the infectious wastes in order to achieve better autoclave sterilization effect.Methods In the autoclave cycles P4 and P9,different packing layer (single,double and triple) and sealing mode (slightly folded,autoclave tape,nylon tape closure) were applied to the internal infectious waste sterilization,biological indicators were used to detect the effect of autoclave sterilization of the upper,middle and lower layers,respectively.Results 1) In the autoclave cycle P4,the triple autoclave bags packaging sealing method was based on the slight folding,autoclave tape,and nylon taple,the biological indicator negative rate of the internal infectious waste in the upper layer,the middle layer and the lower layer were respectively in the upper layer 100%,0%,0%,in the middle layer 67%,20%,0%,and in the lower layer was 0%.The double autoclave bags packaging sealing method was based on the slight folding,autoclave tape,and nylon taple,the biological indicator negative rate of the internal infectious waste in the upper layer,the middle layer and the lower layer were respectively the in the upper layer 100%,85%,0%,the middle layer 45%,0%,0%,and the lower layer 10%,0%,0%.the single autoclave bag packaging sealing method wa based on the slight folding,autoclave tape,and nylon taple,the biological indicator negative rate of the internal infectious waste in the upper,middle and lower layers were respectively the upper layer 100%,100%,0%,the middle layer 70%,45%,0%,and the lower layer 12.5%,0%,0%.(2) In the autoclave cycle P9,the triple autoclave bags packaging sealing method was based on the slight folding,autoclave tape,and nylon taple,the biological indicator negative rate of the internal infectious waste in the upper layer,middle and lower layers were respectively the upper layer all 100%,the middle layer 100%,100%,70%,and the lower layer 100%,100%,30%.The double autoclave bags packaging sealing method was based on the slight folding,autoclave tape,and nylon taple,the biological indicator negative rate of the internal infectious waste in the upper,middle and lower layers were respectively the upper layer all 100%,the middle layer all 100% and the lower layer is 100%,100%,and 60%.The single autoclave bag packaging sealing method was based on the slight folding,autoclave tape,and nylon taple,the biological indicator negative rate of the internal infectious waste in the upper,middle and lower layers were all 100%.Conclusions When autoclave is recommended for sterilization of infectious waste,the best effect was achieved with a single unsealed autoclave bag.If it is necessary to seal,the autoclave bag should not be completely sealed,avoiding incomplete penetration of steam into the interior space,otherwise the infectious waste can not be completely sterilized.