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老年骨髓增生异常综合征患者血CD4^+T细胞中铁过载程度与细胞增殖和分化的关键转录因子的关系 被引量:3

Relationship between the severity of iron overload in peripheral blood CD4^+T cells and the key transcription factors of RORrt, IRF8, STAT3 for cell proliferation and differentiation in elderly patients with myelodysplastic syndrome
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摘要 目的观察外周血CD4^+T细胞内维A酸相关孤儿受体γt(R(ORrt)、干扰素调节因子8(8RF8)、磷酸化信号转导和转录激活因子3(p-STAT3)与老年患者铁过载骨髓增生异常综合征(MDS)的关系。方法前瞻性病例对照研究,人选2017年1月至2018年12月因MDS铁过载住院老年患者22例(观察组),年龄60?78岁,男性12例,女性10例。同期住院MDS非铁过载患者20例为对照组.健康老年人26例为健康组。收集入选者新鲜的外周全血以制备单核细胞.通过流式细胞术分选CD4^+T细胞,并通过实时荧光定量反转录聚合酶链反应(qRT-PCR)和免疫印迹检测RORrt、p-STAT3及IRF8转录因子的表达水平。结果qRT-PCR结果提示,观察组患者RORrt和p-STAT3转录因子在外周血CD4^+T细胞表达分别为42.634+18.613和29.710+9.689,均高于对照组的21.289+15.158和12.355+4.681(均P<0.01),也高于健康组的22.520+9.896和9.818±3.845(均P<0.01),IRF8水平为19.293±8.258,均低于对照组23.785±12.498和健康组69.619+23.768(均PV0.01);免疫印迹检测结果显示,观察组患者外周血CD4^+T细胞RORrt和P-STAT3蛋白水平高于对照组患者和健康组人群(均P<0.01),观察组外周血CD4^+T细胞IRF8蛋白表达低于对照组患者和健康组人群(均P<0.01)。结论CD4^+T细胞内RORrt、IRF8和P-STAT3转录因子水平异常对老年MDS患者铁超载的发病机制中起到积极作用。 Objective To investigate the effects RORrt(RORrt), IRF8 (IRF8 ) and STAT3 (STAT3) in peripheral blood CD4^+T cells on the cell proliferation and differentiation in elderly patients with iron-overload myelodysplastic syndrome( MDS).Methods A prospective case-control study was conducted.Twenty-two elderly hospitalized patients(12 males and 10 females) aged 60-78 years with iron-overload MDS from Jan.2017 to Dec.2018 were enrolled and considered as the observation group.Twenty MDS patients without iron overload hospitalized in the same period were selected as the non-iron overload group, and 26 healthy elderly people were considered as the healthy control group.Peripheral blood monocytes( PBM) were prepared and resident CD4^+T cells were sorted by flow cytometry.The mRNA and protein expression levels of transcription factors of RORrt, p-ST AT 3 and IRF8 were detected by quantitative real time polymerase chain reaction (qRT-PCR) and Western blotting.Results In peripheral blood CD4^+T cells, the mRNA expression level of RORrt and p-STAT3 were higher and that of IRF8 was lower in the iron-overload group than in the non-iron overload group and the healthy control group (42.634±18.613 vs.21.289±15.158 and 22.520±9.896;29.710±9.689 vs.12.355±4.681 and 9.818±3.845;19.293±8.258 vs.23.785±12.498 and 69.619±23.768 ,P<0.01).In peripheral blood CD4^+T cells, the protein expression level of RORrt and p-STAT3 was higher and that of IRF8 was lower in the iron overload group than in the non-iron overload group and healthy control group(P<0.01).Conclusions The abnormalities of the mRNA and protein expression levels of transcription factors of RORrt, IRF8 and p-STAT3 in CD4^+T cells play a fundamental role in the pathogenesis of iron overload MDS in elderly patients.
作者 李康保 李庆山 程艳华 杜庆华 Li Kangbao;Li Qingshan;Cheng Yanhua;Du Qinghua(Department of Geriatrics Blood and Oncology , Guangzhou First People's Hospital, Guangzhou 510180 ,China)
出处 《中华老年医学杂志》 CAS CSCD 北大核心 2019年第3期229-232,共4页 Chinese Journal of Geriatrics
关键词 骨髓增生异常综合征 抗原 CD4 干扰素调节因子类 Myelodysplastic syndrome Antigens, CD4 Interfer on regulatory factors
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