MDI职业暴露员工外周血淋巴细胞亚群水平检测分析

Detection and analysis of peripheral blood lymphocyte subsets in MDI occupational exposure workers

目的探讨二苯基甲烷二异氰酸酯职业暴露员工外周血淋巴细胞亚群各项指标水平的变化.方法将100例在聚氨酯生产行业工作的员工设为观察组,按照工龄中位数分为短时间组(工龄≤8 a)55例及长时间组(工龄>8 a)45例;选取同期同公司未接触MDI员工100例设为对照组.检测所有员工外周血淋巴细胞总数,自然杀伤细胞、CD3^+T细胞、CD4^+T细胞、CD8^+T细胞、B细胞、淋巴细胞水平,对测定结果进行对比分析.结果观察组自然杀伤细胞、CD3^+T细胞、CD4^+T细胞、CD8^+T细胞、B细胞、淋巴细胞水平均显著高于对照组(P<0.01);观察组职业不同暴露时间员工长时间组外周血淋巴细胞亚群各项指标水平虽高于短时间组,但差异均无统计学意义(P>0.05).结论二苯基甲烷二异氰酸酯职业暴露员工外周血淋巴细胞亚群各项指标水平显著高于未接触者.

Objective To explore the changes in the levels of peripheral blood lymphocyte subsets in MDI occupational exposure workers.Methods 100 workers working in the polyurethane production industry were selected as the observation group.According to the length of service,they were divided into short time group(working age≤8 a,55 cases)and long time group(working age>8 a,45 cases).Another 100 employees without contact with MDI and working in the same company were selected as the control group.The total number of peripheral blood lymphocytes of all employees,natural killer cells,CD3^+T cells,CD4^+T cells,CD8^+T cells,B cells,and lymphocyte levels were measured,and the results were compared and analyzed.Results levels of NK cells,CD3^+T cells,CD4^+T cells,CD8^+T cells,B cells and lymphocytes were higher in the observation group than those in the control group(P<0.01),and higher in the long time group than those in the short time group,the difference was not statistically significant(P>0.05).Conclusion The levels of peripheral blood lymphocyte subsets in MDI occupational exposure workers were significantly higher than those in non-contact groups.