1-溴丙烷中毒患者治疗前后蛋白质谱的变化

Changes in mass spectrometry of proteins in patients with 1-bromopropane poisoning after treatment

目的探索1-溴丙烷(1-BP)中毒患者治疗前后蛋白质图谱的变化以及其生物学功能。方法于2016年5月至2017年12月,收集深圳市宝安区3例1-BP中毒患者,均为男性,年龄31~47岁,采集其治疗前后的全血样本2ml。采用蛋白质组学非标Labelfree质谱分析技术进行蛋白鉴定和定量,对两组样本之间差异表达的蛋白质进行分析,应用生物信息学工具分析筛选到的蛋白质功能及其参与的生物过程。结果蛋白组学分析显示,1-BP中毒患者治疗后差异表达超过2倍且P<0.05的蛋白有47个,其中27个在1-BP中毒患者治疗后血清中表达上调,20个在治疗后表达下调;筛选到的蛋白质主要涉及蛋白水解、蛋白修饰、免疫反应、补体激活、脂蛋白代谢过程、信号转导、凝血反应等功能。结论患者治疗前后差异表达的蛋白质有助于1-BP中毒的诊断、治疗和预后监测,可为1-BP中毒治疗提供潜在的疗效和预后的标志物。

Objective To investigate the changes in mass spectrometry of proteins in patients with 1-bromopropane (1-BP) poisoning after treatment and their biological functions. Methods From May 2016 to December 2017, 3 male patients aged 31-47 years with 1-BP poisoning in Bao'an District of Shenzhen, China were enrolled in this study. The whole blood sample (2 ml) was collected before and after treatment. Label-free mass spectrometry-based proteomics was used for protein identification and quantification. The differentially expressed proteins after treatment were analyzed. Bioinformatics tools were used to analyze the functions of the identified proteins and the biological processes they were involved in. Results Proteomic analysis showed that there were 47 proteins that were differentially expressed more than 2-fold (P<0.05) after treatment in the patients with 1-BP poisoning;of them, 27 were up-regulated and 20 were down-regulated in the serum of treated patients. The identified proteins were mainly involved in proteolysis, protein modification, immune response, complement activation, lipoprotein metabolism, signal transduction, and coagulation. Conclusion The differentially expressed proteins after treatment can help with the diagnosis, treatment, and prognosis monitoring of 1-BP poisoning and provide potential therapeutic and prognostic markers for 1-BP poisoning treatment.