分次注射对裸鼠脂肪颗粒组织移植的影响

Effects of divided injection on the survival of fat graft in nude mice

目的探讨分次注射对裸鼠脂肪颗粒移植效果的影响。方法用注射器法从健康女性腹部抽取脂肪颗粒组织,以裸鼠为实验对象。对照组于裸鼠皮下注射1次0.5 ml脂肪组织;7 d组、14 d组、30 d组,先注射0.25 ml,随后在7 d、14 d、30 d补充注射0.25 ml脂肪组织。所有动物于首次注射3个月后取材,对取材组织进行湿重测量、HE染色与周脂素免疫荧光观察,评估移植物的保留率与效果。结果各组之间脂肪湿重及体积比较,差异无统计学意义(P>0.05)。7 d组囊肿/液泡成分评分相与其他组比较,差异有统计学意义(P<0.05)。7 d组与14 d组纤维结缔组织占位评分与对照组比较,差异有统计学意义(P<0.05)。各组活性脂肪细胞所占比例:对照组(9.23±8.56)%,7 d组(50±24.57)%,14 d组(12.30±8.23)%,30 d组(21.40±8.34)%,7 d组脂肪细胞所占比例和对照组与14d组比较,差异有统计学意义(P<0.05)。结论在首次脂肪颗粒细胞注射移植后7 d,再补充注射可提高移植物中活性脂肪组织的比例。

Objective To explore the effect of divided injection of fat grafting and to provide a new sight for the strategy of clinical particle fat transplantation. Methods Adipose tissue was aspirated from the healthy female abdomen by liposuction. In the control group, 0.5 ml of adipose tissue was subcutaneously injected into the nude mice. The experimental group was injected with 0.25 ml first, followed by 0.25 ml injection of adipose tissue on the 7th, 14th and 30th days. To assess graft retention rate and effectiveness we measured the wet weight and observed the pathological sections with HE or perilipin immunofluorescence. Results The wet weight of the tissue between the experimental group and the control group had no statistical difference, the experimental group had less necrosis and empty tissue than the control group. The proportion of perilipin positive staining tissue in 7 day group had statistical difference from that of the control group. Conclusions The strategy of pre-injection of part of the adipose tissue and then supplement of the residual tissue after 7 days may increase the proportion of active adipose tissue in the graft.