摘要
Objective To determine the level of soluble B7-H1(s B7-H1)in serum of patients with colorectal cancer(CRC),and to investigate its clinical application value in CRC.Methods 152 cases of CRC,57 cases of benign colorectal diseases and 59 healthy subjects were enrolled.ELISA was used to determine the s B7-H1 level in serum.The levels of carcinoembryonic antigen(CEA),carbohydrate antigen 19-9(CA19-9)and carbohydrate antigen 72-4(CA72-4)were determined by electrochemiluminescence.The level of carbohydrate antigen 50(CA-50)was determined by chemiluminescence.ROC curve was used to evaluate the diagnostic efficiency of s B7-H1 alone or combined with other tumor markers in CRC.It also analyzed the correlation between serum s B7-H1 level and some clinicopathological characteristics including tumor location,depth of invasion,lymph node metastasis status,distant metastasis status and tumor stages.Results(1)The differences of s B7-H1 level among CRC group,colorectal benign disease group and healthy control group(compared with each other respectively)were statistically significant(P<0.001).Levels of CEA,CA19-9,CA72-4 and CA-50 were significantly different between CRC group and benign colorectal disease group(P<0.05),while there was no significant difference between the benign colorectal disease group and the healthy control group(P>0.05).(2)The diagnosis ability of s B7-H1,CEA,CA19-9,CA72-4,and CA-50 were determined by the area under curve(AUC),which was 0.730,0.772,0.639,0.663 and 0.635,respectively.The combination of s B7-H1 and CEA showed the best effect,and the AUC reached 0.831.(3)There was no significant correlation between the levels of s B7-H1 and CEA,or CA19-9,CA72-4,CA-50.(4)Immunohistochemistry result showed that the positive rate of CRC group was 52%,while that of benign disease group was 6.3%.The difference of the positive rate between the two groups was statistically significant(P<0.01).And there was a significant correlation between the positive rate of s B7-H1 level in serum and tumor tissues of CRC patients(P<0.05).(5)The serum s B7-H1 level in CRC patients was related to lymph node metastasis status(P<0.05).But there was no relationship between s B7-H1 level and other clinicopathological characteristics(including tumor location,depth of invasion,distant metastasis status and tumor stage)(P>0.05).Conclusion Serum s B7-H1 has a high clinical application value and could serve as a new tumor marker in CRC.
作者
XU Minyi
徐旻一(Dept Clin Lab,Shanghai 8th People's Hosp,Xuhui Branch,Shanghai 6th People's Hosp,Shanghai 200235)
