Experimental study of regulatory effect ofhydrogen sulfide on s Flt-1 production in adipocytesthrough ADAM17

Objective To study the effect of hydrogen sulfide on the production of soluble fms-like tyrosine kinase 1(s Flt-1)through a distintegrin and metalloproteinase 17(AD-AM17)in adipocytes.Methods 3T3-L1 cells were cultured and induced to differentiate into adipocytes,then treated with different doses of sodium hydrogen sulfide(NaHS),L-cysteine or transfected with cystathionine-γ-lyase(CSE)siRNA,ADAM17 siRNA or treated with ADAM17 inhibitor,monoclonal antibody.24 hours after treatment,the expression of ADAM17,CSE,and the production of s Flt-1 were determined.Results After the treatment of 10,25,50 nmol/L NaHS or 0.5,1.0,2.0 μmol/L L-cysteine,the expression of ADAM17 and the production of s Flt-1 in adipocytes were significantly decreased,the higher does of L-cysteine and s Flt-1,the lower expression of ADAM 17 and the production of s Flt-1;the effect of 2.0 μmol/L L-cysteine decreasing the expression of ADAM 17 and the production of s Flt-1 were reversed by transfection of CSE siRNA;after the transfection of ADAM17 siRNA and treatment of ADAM17 inhibitor or monoclonal antibody,the production of s Flt-1 in adipocytes were significantly decreased.Conclusion Hydrogen sulfide can reduce the production of s Flt-1 in adipocytes by downregulating the expression of ADAM17.