HPLC同时测定退黄保肝胶囊中3种成分的含量

Simultaneous Determination of Three Components in Tuihuang Baogan Capsules by HPLC

目的建立HPLC测定退黄保肝胶囊中绿原酸、柚皮苷及新橙皮苷含量的方法。方法色谱柱:InertSustain C_(18) Superb(4.6 mm×250 mm,5μm);流动相:乙腈-0.2%磷酸溶液(15:85);流速:1.0 ml/min;检测波长283 nm;柱温:35℃。结果 3种成分的线性关系均良好,绿原酸回归方程为Y=2×10~6X-523,r=0.9999,柚皮苷回归方程为Y=2×10~6X+388,r=0.9999,新橙皮苷回归方程为Y=2×10~6X+436,r=0.9996,回收率分别99.0%,99.3%,100.1%,RSD均低于2.0%。结论本方法简单快速,适用于测定退黄保肝胶囊中的绿原酸、柚皮苷和新橙皮苷的含量。

Objective To establish a method for simultaneous determination of chlorogenic acid,naringin and neohesperidin in Tuihuangbaogan Capsules by HPLC.Methods InertSustain C18 Superb(4.6 mm×250 mm,5μm)was adopted,using acetonitrile-0.2%phosphoric acid solution(15:85)as mobile phase,with a flow rate of 1.0 ml/min.The detection wavelength was set at 283 nm;the column temperature was 35℃.Results The linear relationships of the three components were good.The regression equation of chlorogenic acid was Y=2×10^6X-523,r=0.9999;the regression equation of naringin was Y=2×10^6X+388,r=0.9999;and the regression equation of neohesperidin was Y=2×10^6X+436,r=0.9996.The recoveries were 99.0%,99.3%and 100.1%,respectively.The RSDs were lower than 2.0%.Conclusion The method is simple and rapid,and can be used to determine the contents of chlorogenic acid,naringin and neohesperidin in the Tuihuangbaogan Capsules.