翅碱蓬响应高盐胁迫的分子机制研究

Study on the molecular mechanisms of Suaeda heteroptera in response to high salt stress

为研究翅碱蓬Suaeda heteroptera响应高盐胁迫的分子机制,试验将翅碱蓬植株在高盐胁迫(856 mmol/L NaCl)和无盐(0 mmol/L NaCl)条件下处理2 h后,对植株分别进行全转录组测序。结果表明:通过RNA-seq分析共获得转录本147 176个,拼接后获得单基因(Unigene)84 545个;比较转录组学分析显示,与无胁迫组(对照)相比,高盐胁迫下翅碱蓬共检出144个差异表达基因,其中发生表达上调的有55个,发生表达下调的有89个;GO富集分析显示,差异表达基因主要分布在次生代谢、信号转导、光合作用电子传递等途径;KEGG富集分析显示,差异表达基因涉及的代谢通路有14个;对差异表达基因发生富集的5个主要代谢通路(光合作用、蔗糖与海藻糖代谢、植物激素信号转导、苯丙基类生物合成、半胱氨酸与甲硫氨酸代谢)进行了详细阐述,从分子水平探讨了翅碱蓬响应高盐胁迫的机制。本研究结果可为更深入研究翅碱蓬耐盐分子机制、挖掘耐盐关键基因提供数据资料。

In order to study the molecular mechanisms of Suaeda heteroptera in response to high salt stress,the plants were divided into two groups,the salt stress group(856mmol/L NaCl)and the control group(0mmol/L NaCl).For each group,total RNA was isolated after 2 h treatment and subject to comparative transcriptomic analysis.In total,147 176 transcripts were obtained from RNA-seq and 84 545 unigenes were obtained after splicing.The results showed that 144 differentially expressed genes(DEGs)were detected in Suaeda heteroptera under high salt stress,of which 55 were up-regulated and 89 were down-regulated.GO enrichment analysis showed that these DEGs were mainly distributed in secondary metabolism,signal transduction,photosynthesis electron transport and other pathways.KEGG enrichment analysis of DEGs showed that there were 14 metabolic pathways involved.Five main metabolic pathways of DEGs enrichment were discussed in detail,including photosynthesis,sucrose and trehalose metabolism,plant hormone signal transduction,phenylpropyl biosynthesis,cysteine and methionine metabolism.The molecular mechanisms of Suaeda heteroptera to high salt stress were discussed.This study could provide data for further the study of salt tolerance in Suaeda heteroptera and relevant genes identification.