超声辅助双水相提取油菜籽粕黄酮及抗氧化性研究

Ultrasonic-assisted Aqueous Two-phase Extraction of Total Flavonoids from Rapeseed Meal and Its Antioxidant Activity

在单因素试验的基础上结合正交试验,采用超声辅助乙醇-硫酸铵双水相提取油菜籽粕黄酮,并考察其抗氧化活性。研究结果表明,超声辅助双水相提取油菜籽粕黄酮的最优工艺为:硫酸铵浓度0.3g/mL,提取温度40℃,提取时间30min,料液比1∶30(g/mL),超声功率280W,在该工艺条件下,油菜籽粕黄酮的含量为(5.53±0.03)mg/g。体外抗氧化活性研究表明,油菜籽粕黄酮提取液对·OH和O2-·均具有一定的清除活性,并且其含量与抗氧化活性具有一致性。说明油菜籽粕黄酮是一种天然抗氧化活性剂和自由基清除剂。

The ultrasonic-assisted aqueous two-phase system is adopted to extract total flavonoids from rapeseed meal and its antioxidant activity is studied.The extraction process is optimized by singe factor test and orthogonal test methods,the total flavonoids yield is as the index.The results show that the optimum extraction process is determined as follows:ammonium sulfate concentration is 0.3 g/mL,ultrasound temperature is 40℃,ultrasound time is 30 min,solid-liquid ratio is 1∶30(g/mL),ultrasound power is 280 W.By adopting the process,the extraction yield of flavonoid is(5.53±0.03)mg/g.Comparing with the conventional antioxidant ascorbic acid,the extraction of flavonoid from rapeseed meal in vitro could be better removing hydroxyl free radical(·OH)and superoxide anion radical O 2^-·,which is consistent with its content.Conclusion:Flavonoid obtained in rapeseed meal is a natural antioxidant and free radical scavenger.