酸枣多糖铁药理活性初步研究

Preliminary Study on Pharmacological Activity of Ziziphus jujuba Mill. var.spinosa (Bunge) Hu ex H.F.Chow Polysaccharide-Fe (Ⅲ) Complex

目的:评价酸枣多糖铁(ZJSP-Fe(Ⅲ))抗缺铁性贫血作用,为酸枣资源开发利用提供依据。方法:采用紫外、红外、扫描电镜等方式对其形态及微观结构进行表征,采用1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2-联氮基双(3-乙基-苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基以及OH自由基对其清除率进行抗氧化作用研究,对于其体外铁释放率采用人工胃液及肠液进行研究,抗缺铁性贫血作用采取缺铁性贫血大鼠模型进行研究。并比较给药前后大鼠血常规数据及血清中促红细胞生成素(EPO)以及Hepcidin含量,判断其改善IDA大鼠模型的效果。结果:合成的酸枣多糖铁中铁含量可达21.7%,对DPPH·、ABTS^+·及·OH清除率的EC_(50)分别为1.47、10.21、0.37 mg/mL,在人工消化液中保持3 h后释铁率可达89.28%,合成的酸枣多糖铁能够显著(p <0.05)增加缺铁性贫血大鼠模型红细胞数量(RBC)、血红蛋白含量(HGB)、红细胞压积(HCT)、EPO、Fe等指标,可显著(p <0.05)降低Hepcidin水平。结论:酸枣多糖铁具有良好的抗氧化活性及体外释铁性能,能够有效改善IDA大鼠模型缺铁症状,有望作为一种新型功能性补铁剂进行继续研究,并需进一步对其抗缺铁性贫血机理进行研究。

Objective: To evaluate the anti-iron deficiency anemia effect of Ziziphus jujuba Mill. pollysaccharide-iron complex (ZJSP-Fe (Ⅲ)),to provide basis for development and utilization of wild jujube resources.Methods: The UV,FT-IR,SEM were used to characterize the ZJSP-Fe (Ⅲ),DPPH·,ABTS+· and ·OH scavenging test were used to investigate the antioxidant effect,the iron release effect was tested by artificial gastric juice and intestinal fluid,the iron deficiency model was used to investigate the anti-IDA effect by comparing the data about routine blood test,the contents of EPO and hepcidin before and after administration.Results: The iron contents in ZJSP-Fe (Ⅲ) was 21.7%,and the EC50 of scaveng rates for DPPH·,ABTS+·and·OH were 1.47,10.21,0.37 mg/mL,after digested in artificial gastric juice and intestinal fluid the iron release rate was 89.28%,the RBC,HGB,HCT,MCV level in IDA rats were ameliorated after administration of ZJSP-Fe (Ⅲ).Conclusion: ZJSP-Fe (Ⅲ) showed good antioxidant and iron release effect in vitro (p < 0.05),and it could ameliorate the IDA symptom (p < 0.05),which would be a new iron supplement,and necessary to investigate the mechanism.