摘要
本文通过细胞荧光强度、3种抗氧化酶活性(超氧化物岐化酶,SOD;谷胱甘肽过氧化物酶,GSH-Px;过氧化氢酶,CAT)、谷胱甘肽(GSH)和丙二醛(MDA)含量的测定,在细胞模型和动物实验中研究γ-萜品烯的体内外抗氧化能力。结果表明,Hep-G2细胞内的荧光强度随着γ-萜品烯质量浓度的增加而下降,二者呈现良好的剂量-作用关系,说明γ-萜品烯可以有效结合细胞膜内外的自由基或活性氧,从而减少细胞内带有荧光的氧化产物的积累;过氧化氢诱导建立的氧化应激细胞模型和动物实验结果表明,γ-萜品烯可以显著提高超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)的活性及谷胱甘肽的含量(p <0.05),并显著降低丙二醛的含量(p <0.05)。以上研究结果表明,γ-萜品烯在体内外均表现出一定的抗氧化作用。
In order to verify the antioxidant activity of γ-terpinene,a main component of cumin essential,fluorescence intensity,three antioxidases (superoxide dismutase,SOD;glutathione peroxidase,GSH-Px;catalase,CAT) activities,and contents of glutathione (GSH) and malondialdehyde (MDA) were evaluated by cell test and animal study.The results showed that fluorescence intensity in Hep-G2 cell was decreased by γ-terpinene with dose-dependent,which demonstrated that oxygen radical was bonded to γ-terpinene both inside and outside the cells,thus,less oxidative products with fluorescence were accumulated in cells.In the experimental model of oxidative stress induced by H2O2 and the animal study,γ-terpinene could significantly increase the activities of antioxidant enzymes (SOD,GSH-Px,CAT) and content of GSH (p < 0.05).On the other hand,content of MDA was decreased significantly (p < 0.05),which was similar to the results in vivo. Above all,γ-terpinene presented antioxidant activity both in vitro and vivo.
作者
甘芝霖
倪元颖
GAN Zhi-lin;NI Yuan-ying(Department of Food Science and Engineering, College of Biological Sciences and Technology,Beijing Forestry University,Beijing 10083 ,China;College of Food Science and Nutritional Engineering,China Agricultural University, Beijing 10083 , China)
出处
《食品工业科技》
CAS
北大核心
2019年第6期100-105,113,共7页
Science and Technology of Food Industry
基金
国家自然科学基金面上项目(31171675)
