双歧杆菌复合微胶囊的工艺优化、表征及功能特性分析

Process Optimization,Characterization and Functional Properties Analysis of Bifidobacterium Composite Microcapsules

为提高动物双歧杆菌BB12(Bifidobacterium animalis subsp.lactis BB12)的稳定性,以香蕉皮水不溶性膳食纤维-海藻酸钠-卡拉胶复合材料为壁材制备双歧杆菌微胶囊。在单因素实验和爬坡实验的基础上,以包埋产率为响应值,应用Box-Behnken试验设计建立回归模型并进行方差分析。通过结构表征、模拟消化环境对微胶囊的特性进行综合评价。结果表明,制备双歧杆菌微胶囊的优化工艺条件为:香蕉皮水不溶性膳食纤维含量46.00%、海藻酸钠浓度2.70%、卡拉胶5.60%、低聚果糖2.60%、搅拌时间31.00 min,此条件下,微胶囊的包埋产率为73.02%。红外光谱分析表明,双歧杆菌包埋在复合壁材中,并形成微胶囊;扫描电镜显示微胶囊内部疏松多孔。制备的双歧杆菌微胶囊在模拟胃液、高胆汁盐溶液中均具有较好的耐受性。本研究为水不溶性膳食纤维和益生菌微胶囊的进一步开发利用奠定基础。

To improve the stability of Bifidobacterium animalis subsp.lactis BB12,Bifidobacterium microcapsules were prepared using water-insoluble dietary fiber in banana peel-sodium alginate-carrageenan composites as wall materials. Based on the single-factor experiment and hill-climbing test,embedded yield was used as the response value,and Box-Behnken test was used to establish a regression model for variance analysis.The characteristics of the microcapsules were evaluated by structural characterization and simulated digestion conditions. The results indicated that the optimized conditions for preparation of Bifidobacterium microcapsules were as follows: Content of water-insoluble dietary fiber in banana peel was 46.00%,concentration of sodium alginate was 2.70%,concentration of carrageenan was 5.60%,concentration of Fructo oligosaccharides was 2.60%,and stirring time was 31.00 min. The embedded yield under the optimized conditions was 73.02%. Infrared spectroscopy analysis showed that Bifidobacterium was embedded in the composite wall material and microcapsules were formed.In addition,the inside of the microcapsule was loose and porous,as observed under scanning electron microscopy. The prepared bifidobacterial microcapsules had good tolerance in the conditions of simulating gastric juice and high bile salt solution.This study laid the foundation for further development and utilization of water-insoluble dietary fiber and probiotic microcapsules.