刺参粗多糖对糖酵解与有氧氧化过程关键酶活性的影响

Effects of Stichopus japonicus Crude Polysaccharides on the Key Enzymes in Glycolysis and Aerobic Oxidation

目的:研究刺参粗多糖(Stichopus japonicus crude polysaccharides,SJP)对糖酵解与氧化磷酸化关键酶的作用。方法:采用酶解法(木瓜蛋白酶、胃蛋白酶与胰蛋白酶)提取SJP。实验小鼠分为正常对照组、SJP 1组(木瓜蛋白酶水解法制备的SJP)、SJP 2组(胃蛋白酶与胰蛋白酶水解法制备的SJP),每组8只小鼠,灌胃剂量为200 mg/kg/d,每日上午九点灌胃,每日1次,给药10 d,正常对照组小鼠每天灌胃等体积生理盐水。测定刺参粗多糖对糖酵解过程关键酶己糖激酶(HK)、3-磷酸甘油醛脱氢酶(GAPDH)、乳酸脱氢酶(LDH)与有氧氧化过程关键酶丙酮酸脱氢酶(PDH)、线粒体复合体Ⅰ、线粒体复合体Ⅴ的影响。结果:与对照组相比,SJP组小鼠的HK、GAPDH及LDH活性均下降,且存在显著性差异(p <0.05); SJP组小鼠的PDH、线粒体复合体Ⅰ及复合体Ⅴ活性均升高,并存在显著性差异(p <0.05),即SJP可抑制HK、GAPDH及LDH的活性,促进PDH、线粒体复合体Ⅰ及线粒体复合体Ⅴ的活性。结论:SJP可抑制糖酵解促进有氧氧化过程,而癌细胞的主要特征是通过糖酵解产生能量,进而推测SJP具有预防癌症的作用。

Objective: To study the effects on key enzymes glycolysis and oxidative phosphory-lation of Stichopus japonicus crude polysaccharides (SJP). Methods: SJP were extracted by enzymatic hydrolysis (papain,pepsin and trypsin),the experimental mice were divided into normal control group,SJP 1 group (papain hydrolysis method prepared by SJP),SJP 2 group (pepsin and trypsin hydrolysis method prepared by SJP),8 mice per group. The gastric dose was 200 mg/kg/d for 10 days,administered daily at 9: 00 am,and the normal control mice were given an equal volume of normal saline.The effects of SJP on the glycolytic key enzymes (hexokinase,3-glyceraldehyde phosphate dehydrogenase,lactate dehydrogenase) and aerobic oxidation key enzymes (pyruvate dehydrog-enase,mitochondrial complexⅠ,mitochondrial complex Ⅴ) were determined. Results: Compared with the control group,the activities of HK,GAPDH and LDH in the SJP group were decreased,and there was significant difference (p < 0.05),the activities of PDH,mitochondrial complex I and complex V in SJP were increased,and there was significant difference (p < 0.05),that was SJP could inhibit the activities of hexokinase,3-glyceraldehyde phosphate delydrogenase and lactate dehydrogenase,increased the activities of pyruvate dehydogenase,mitochondrial complex Ⅰ and mitochondrial complex Ⅴ.Conclusion: SJP could inhibit glycolysis and promote the aerobic oxidation.The main characteristic of cancer cells is that the energy is produced through glycolysis,and it is inferred that SJP has a role in preventing cancer.