天降血栓通丸对2型糖尿病大鼠血糖、大血管病变的影响

Clinical effect of Tianjiang Xueshuantong pills on blood glucose and macrovascular complications in rats with type 2 diabetes

目的:研究天降血栓通丸对2型糖尿病大鼠血糖、大血管病变的影响。方法:将90只wistar大鼠随机分为正常组15只与造模组75只,通过高糖高脂饲料+链脲佐菌素诱导建立糖尿病大鼠模型,造模成功后将其随机分为模型组,天降血栓通丸早期干预组、中期干预组、晚期干预组(简称为早期组、中期组、晚期组),盐酸吡格列酮组(西药组),每组各15只,分别采用相应药物干预。观察大鼠一般情况,检测空腹血糖、胰岛素、胰岛素敏感指数、细胞间黏附分子-1(ICAM-1)水平。结果:造模组与空白组比较,胰岛素、胰岛素敏感指标、体质量、第13周空腹血糖差异均有统计学意义(P <0. 01或P <0. 05);药物干预后空腹血糖水平ICAM-1模型组、各治疗组与空白组比较,各治疗组与模型组比较,差异均有统计学意义(P <0. 05)。结论:天降血栓通丸无降糖作用,其早期干预能有效降低ICAM-1,这可能是其对2型糖尿病大鼠大血管病变影响的机制之一。

Objective :To investigate the clinical effect of Tianjiang Xueshuantong pills on blood glucose and macrovascular complications in rats with type 2 diabetes. Methods: A total of 90 Wistar mts were randomly divided into normal group with 15 rats and model group with 75 rats. High -sugar and high- fat feed and streptozotocin were used to establish a rat model of diabetes, and after the model was successfully established, the rats were divided into model group, early -, middle-, and late - stage Tianjiang Xueshuantong pill intenention groups( early -, middle -,and late - stage intervention groups, respectively), and pioglitazone hydrochloride group ( Western medicine group), with 15 rats in each group. The rats were treated with corresponding drugs. General status of the rats was observed ,and fasting blood glucose ( FBG ), insulin, insulin sensitivity index ( ISI ), and intercellular adhesion molecule- 1 (ICAM -1 ) were measured. Results: There were significant differences between the model group and the normal group in insulin, ISI, body weight, and FBG at week 13 ( P < 0. 01 or P < 0. 05 ). After drug intervention, there were significant differences in FBG and ICAM -1 between the model group/treatment groups and the normal group, as well as between the treatment groups and the model group ( P < 0. 05 ). Conclusion : Tianjiang Xueshuantong pills do not have a hypoglycemic effect, and its early intervention can effectively reduce ICAM -1 , which might be one of the mechanisms of action of Tianjiang Xueshuantong pills in the treatment of niacrovascular complications in rats with type 2 diabetes.