即时检测芯片通道表面亲水涂膜处理工艺

Hydrophilic coating treatment process on channel surface of POCT chips

聚合物即时检测(POCT)芯片通道自身表面张力不足,液体在通道中前进会造成前端流动形状不稳定,或引起断流现象,严重影响检测结果。设计并制作即时检测芯片,利用二氧化钛(Ti O_2)亲水浸渍提拉涂膜的方法对芯片通道进行改性研究。研究结果显示,亲水涂层使材料表面羟基含量提高,增强了其亲水性;亲水涂覆后,液体在芯片通道中流动形状与流动速度稳定;亲水膜表面成膜质量较好,成膜厚度在百纳米级,不会影响通道微结构; c Tn I荧光检测峰值稳定。

Because of the weakness of surface tension in channel of polymer microfluidic chip,the unstable flow shape of liquid front-end will occur,even cause cut off which will seriously affect the results of test.A POCT chip is designed and fabricated,and hydrophilic coating of TiO2 method is used to solve the problem.Research result shows that the hydrophilic coating improves the hydroxyl content and enhances hydrophilicity of the material.And the liquid has stable flow shape and velocity in channel of chip after hydrophilic coating.In addition,the hydrophilic film has good quality,and the film thickness can reach the hundred nanometers level which will not affect the microstructures in the channel,and the peak value of cTnI fluorescence detection is stable.