红毛鸭胚源鹅细小病毒江西株的分离鉴定与NS基因的分析

Isolation and identification of goose parvovirus JX-JA-2017 strain from Hongmao duck embryos and Sequence Analysis of NS Gene

自死亡的红毛鸭胚中分离获得1株病毒株(命名为JX-JA-2017株),经胶体金试纸条检测呈鹅细小病毒(GPV)阳性,经中和试验测定可被GPV阳性血清特异性中和,表明该毒株为GPV。对其进行非结构基因(NS)特征分析发现,其NS基因与GenBank登入的13株鹅细小病毒核苷酸序列同源性为94%～99.6%,氨基酸同源性为94.8%～99.5%;经分子进化分析发现,该株病毒与亚洲型GPV分离株共处于一个进化分支。以上结果揭示,红毛鸭胚存在GPV感染,且其JX-JA-2017株病毒与经典的GPV具有共同的遗传起源,丰富了GPV的流行病学与分子生态学内涵,为加强我国鹅细小病毒病的防控提供科学依据。

A goose parvovirus(GPV) strain(named JX-JA-2017) was isolated from dead Jiangxi Hongmao duck embryos and identified by colloidal gold labeled technique, and neutralization test. The colloidal gold test results showed that GPV was positive. The neutralization test results showed that the virus was neutralized by standard positive serum against GPV. The results gene sequences analysis demonstrated that the nucleotide and amino acid homology of NS between JX-JA-2017 strain and other 13 GPV isolates ranged from 94%~99.6% and 94.8%~99.5%. Phylogenetic analysis of NS gene showed that the strain was a distinct lineage of Asian GPV,but was separated from Muscovy duck parvovirus(MDPV).In conclusion, the isolated GPV strains in this study have the same genetic origin as the classical GPV isolates, which provide scientific basis for the prevention and control of parvovirus disease in China.