小鼠内耳发育过程中Evi-1基因的表达

Expression of Evi-1 During Inner Ear Development in Mice

目的观察在小鼠内耳发育过程中不同阶段Evi-1基因的动态表达特征及变化规律,为进一步探索EVI-1基因在小鼠内耳发育中作用。方法选用胚胎第9.5天(E9.5)到胚胎期18.5天(E18.5)的C57BL/6胎鼠,E9.5-E15.5取胚胎头,E15.5后取内耳,之后进行冰冻切片及免疫荧光染色,利用荧光显微镜观察小鼠内耳发育过程中EVI-1基因的表达变化情况。结果在E9.5天时尚未在听泡及其周围组织观察到EVI-1基因的表达,当胚胎发育到E10.5天时,EVI-1开始在感觉上皮周围弱表达,其局限表达于听泡周围的间充质;E11.5时期Evi-1的表达增强但也局限于内耳周围的间充质;E12.5时期时感觉上皮中开始出现Evi-1的表达,但随后即消失,仅在这一时期的感觉上皮中一过性表达,E13.5后,Evi-1表达逐渐减弱至消失。结论 Evi-1基因在小鼠内耳发育时期的表达具有明显时限性以及区域性,可能与内耳感觉细胞的增殖分化有关。

Objective To report dynamic expression characteristics of the Evi-1 gene during different stages of inner ear development. Methods Embryonic C57BL/6 mice ranging from E9.5 to E18.5 were used to observe Evi-1 gene expression during the inner ear development process on serial frozen sections with immunofluorescence staining under a fluorescent microscope. Result Otocyst aroused on E9.5 when there was no visible expression of Evi-1 in the inner ear. The expression of Evi-1 emerged on E10.5 and was localized in periotic mesenchyme during embryonic development from E10.5 to E13.5. Moreover, strong Evi-1 expression was seen not only in mesenchyme by E11.5 but also transiently on sensory epithelium on E12.5. Fluorescence signals gradually decreased after E13.5 and expression of Evi-1 gene in the inner ear and periotic mesenchyme disappeared by E14.5. Conclusion The temporary and regional expression of the Evi-1 gene during the development of the inner ear indicates that Evi-1 may have a significant function during the process of hair cells development.