摘要
目的研究lncRNA FOXD2-AS1对胶质瘤细胞增殖及迁移的作用。方法利用脂质体转染小干扰RNA以沉默lncRNA FOXD2-AS1基因,通过MTT、平板克隆形成实验和transwell小室等实验方法检测胶质瘤细胞的增殖及迁移。结果对照组在96h增殖率为19.8%±1.09%、20.3%±1.12%,沉默lncRNA FOXD2-AS1基因后的增殖率为7.59%±0.615%、17.9%±0.720%,差异有统计学意义。对照组的克隆形成数为119.3±11.0、162.3±50.2,沉默lncRNA FOXD2-AS1基因后克隆形成数为90.33±7.51、21.00±2.65,差异有统计学意义。对照组的细胞迁移数为134.33±32.72、377.67±61.51,沉默lncRNA FOXD2-AS1基因后细胞迁移数为43.000±15.13、196.67±23.46,差异有统计学意义。结论 lncRNA FOXD2-AS1在胶质瘤细胞中可能起促癌作用,促进胶质瘤细胞的增殖及迁移。
Objective This study was performed to reveal the effect of lncRNA FOXD2-AS1 in glioma cells proliferation and migration.Methods The siRNA(si-FOXD2-AS1)was transfected using Lipofectamine 2000 to silence the lncRNA FOXD2-AS1.Then,the proliferation and migration of glioma cells were measured using MTT,colony formation and transwell assay.Results The proliferation rates of control group glioma cells were 19.8%±1.09% and 20.3%±1.12% while the proliferation rates of cells silenced lncRNA FOXD2-AS1 were 7.59%±0.615%and 17.9%±0.720% after transfection for 96 h,the difference was statistically significant.The numbers of colonies in control group were 119.3±11.0 and 162.3±50.2.After silencing the lncRNA FOXD2-AS1,the numbers of colonies were 90.33±7.51 and 21.00±2.65,the difference was statistically significant.The number of cells migration in control group were 134.33±32.72 and 377.67±61.51,the number of cells migration were 43.000±15.13 and 196.67±23.46 after silencing the lncRNA FOXD2-AS1,the difference was statistically significant.Conclusion We proposed that lncRNA FOXD2-AS1 may function as an oncogene and promote the proliferation and migration of glioma cells.
作者
上官文兵
李朝晖
张佳琦
韦博
SHANG-GUAN Wen-bing;LI Zhaohui;ZHANG Jia-qi(College of Life Science Zhejiang Chinese Medical University , Hangzhou 310052 , China)
出处
《中国实验诊断学》
2019年第3期525-527,共3页
Chinese Journal of Laboratory Diagnosis