苏打盐碱胁迫对紫花苜蓿叶片渗透调节和膜透性的影响

Effects of soda saline-alkali stress on osmotic regulation and membrane permeability of Medicago sativa leaves

为了探究苏打盐碱对紫花苜蓿幼苗叶片渗透调节物质和膜透性物质累积的影响,采用不同浓度的苏打盐碱对紫花苜蓿幼苗进行胁迫处理,测定苜蓿叶片的可溶性糖、可溶性蛋白和脯氨酸、MDA含量以及相对电导率的变化.结果表明,可溶性糖、可溶性蛋白和脯氨酸含量均随苏打盐碱浓度的增加呈先升高后降低的变化趋势,在150mmol·L^(-1)处理组达到最高,MDA含量和相对电导率随苏打盐碱浓度的增加呈逐渐上升的变化趋势,在250mmol·L^(-1)处理组达到最高.隶属函数综合分析,苏打盐碱浓度在150mmol·L^(-1)时,渗透调节物质大量积累,生物膜损伤最小.

In order to explore the effects of soda saline-alkali on osmotic adjustment substances and membrane permeability substances accumulation in Medicago sativa seedlings,the experiment was conducted to stress Medicago sativa seedlings with different concentrations of soda saline-alkali,and to determine the changes of soluble sugar,soluble protein,proline content,MDA content and relative conductivity in alfalfa leaves.The results showed that the contents of soluble sugar,soluble protein and proline increased firstly and then decreased with the increase of soda saline-alkali concentration,and reached the highest level in 150 mmol·L^-1 treatment group.The content of MDA and relative conductivity increased gradually with the increase of soda concentration,and reached the highest level in 250 mmol·L^-1 treatment group.According to the comprehensive analysis of membership function,when the soda saline-alkali concentration was 150 mmol·L^-1,osmotic adjustment substances accumulated in large quantities and biofilm damage was the smallest.