PTPN6基因对人食管鳞状细胞癌Eca109和Yes-2细胞恶性生物学行为的影响

Effects of PTPN6 on malignant biological behaviors of human esophageal squamous cancer Eca109 and Yes-2 cells

目的:探讨非受体型蛋白酪氨酸磷酸酶6(non-receptor protein tyrosine phosphatase 6,PTPN6)基因在不同食管鳞状细胞癌细胞株中的表达及其对Eca109、Yes-2细胞恶性生物学行为的影响。方法:应用qPCR法检测不同食管鳞癌细胞株(TE1、Eca109、Kyse150、Kyse170、Yes-2)中PTPN6 m RNA的表达水平,以pcDNA3.1-PTPN6质粒分别瞬时转染食管鳞癌细胞株Eca109和Yes-2,应用qPCR和Wb法检测PTPN6 m RNA和蛋白的表达水平,并应用MTS、克隆形成实验、划痕实验和Transwell法检测过表达PTPN6基因对食管鳞癌细胞恶性生物学行为的影响。结果:PTPN6基因在5种食管鳞癌细胞侏中表达均明显下调(均P<0.05)。与转染空载体的对照组相比,经pcDNA3.1-PTPN6转染后,Eca109和Yes-2细胞均高水平表达PTPN6(P<0.05或P<0.01);过表达PTPN6基因后,Eca109和Yes-2细胞的增殖、迁移和侵袭能力均明显被抑制(均P<0.05)。结论:PTPN6基因高表达能抑制食管鳞癌细胞的增殖、迁移和侵袭,其可能是影响食管鳞癌细胞生物学特性的一个重要因素。

Objective:To detect the expression of non-receptor protein tyrosine phosphatase 6(PTPN6)in different esophageal squamous cell carcinoma(ESCC)cell lines,and to investigate its effect on proliferation,migration and invasion ability of Eca109 and Yes-2 cell lines.Methods:qPCR was applied to detect the mRNA expression of PTPN6 in different ESCC cell lines(TE1,Eca109,Kyse150,Kyse170 and Yes-2).pcDNA3.1-PTPN6 plasmid was transiently transfected into Eca109 and Yes-2 cells respectively.The expression of PTPN6 was detected by real-time PCR and Wb.The effects of PTPN6 over-expression on the biological behaviors of ESCC cells were detected by MTS,colony formation assay,wound healing assay and Transwell assay,respectively.Results:The mRNA expression of PTPN6 was remarkably reduced in ESCC cell lines(TE1,Eca109,Kyse150,Kyse170 and Yes-2)compared to normal esophageal epithelial cells(HEEpiC)(P<0.05).Compared to the mock cells,significant up-regulation of PTPN6 was detected in pcDNA3.1-PTPN6 transfected Eca109 and Yes-2 cells(all P<0.05 or P<0.01);PTPN6 over-expression led to a significant inhibition in migration and invasion ability of Eca109 and Yes-2 cells(all P<0.05).Conclusions:Over-expression of PTPN6 may inhibit the proliferation,migration and invasion of ESCC cells,which might be an important factor influencing the biological characteristics of ESCC cells.