玉米蛋白粉替代鱼粉对暗纹东方鲀消化酶活性的影响

Effect of corn gluten meal partially replaced fish meal on digestive enzymes activities in puffer( Fugu obscurus)

研究了暗纹东方鲀肠、胃和肝胰脏等不同消化器官的消化酶活性,以及不同水平的玉米蛋白粉(0%、5%、10%、15%、20%)对暗纹东方鲀(41. 06±1. 98) g胰蛋白酶、胰凝乳蛋白酶、羧肽酶A、羧肽酶B、胃蛋白酶、氨肽酶以及脂肪酶、淀粉酶活性的影响。结果表明:(1)暗纹东方鲀肠组织中除胃蛋白酶活性显著低于胃组织,其他消化酶活性均显著高于胃和肝胰脏组织(P <0. 05);胃组织中除胃蛋白酶以外其他消化酶活性都较低。(2)玉米蛋白粉对暗纹东方鲀消化酶活性的影响因消化酶的种类各异。胰蛋白酶以10%实验组活性最大;胰凝乳蛋白酶、羧肽酶A、氨肽酶活性随玉米蛋白粉用量的增加而下降,当玉米蛋白粉用量达20%时显著低于对照组(P <0. 05)。胃蛋白酶活性10%实验组显著高于其他各组(P <0. 05);玉米蛋白粉能提高肠和肝胰脏组织脂肪酶和淀粉酶的活性,10%实验组显著高于对照组(P <0. 05)。结论:暗纹东方鲀消化酶活性以肠组织中的最高,胃组织中消化酶活性较弱;玉米蛋白粉显著影响暗纹东方鲀消化酶活性,本实验条件下,玉米蛋白粉用量为10%时暗纹东方鲀消化酶活力最高。

The present study was conducted to determine the activity of enzymes in intestine,stomach,and hepatopancreas of puffer(Fugu obscurus)and whether corn gluten meal(CGM)partially replaced fish meal could affect the activity of trypisn,chymotrypsin,carboxypeptidase A,carboxypeptidase B,pepsin,aminopeptidases,lipase and amylase.Five isonitrogenous and isoenergetic experimental diets were formulated.Diets contained corn gluten meal from 0%to 20%.After 60 d,the activities of the enzymes were measured.The results showed that:(1)The enzymes activity in the intestine were significantly higher than those in stomach and hepatopancreas except pepsin.The enzymes activity of stomach were lower,except pepsin;(2)The corn gluten meal impacts the enzymes activity of Fugu obscurus.Trypsin activity was highest in 10%treatment.The activity of chymotrypsin,carboxypeptidase A and aminopeptidases were linearly decreased with increasing CGM percentage from 5 to 20%,and it was significantly lower than that of the control in 20%treatment.Pepsin activity of 10%treatment was significantly higher than that of other groups.Lipase and amylase activities were increased in the trial groups,and it is significantly higher in 10%treatment than that of the others(P<0.05).It was concluded that the activity of digestive enzymes was highest in intestine,and the upper limit of corn gluten meal for optimal digestive enzymes activity in puffer feed was 10%.