辣根过氧化物酶脂质体电化学生物传感器检测亚硝酸盐

Fabrication of horseradish peroxidase liposome electrochemical biosensor for rapid detection of nitrite

基于静电自组装法,以壳聚糖为介质,利用辣根过氧化物酶的生物反应器修饰玻碳电极,用以构建亚硝酸盐快速检测电化学酶生物传感器。对所制备的辣根过氧化物酶生物反应器的理化性质进行表征;优化了传感检测体系中反应温度、pH、扫描速率和反应时间;采用循环伏安法对修饰电极的电化学性能进行评估。实验结果表明,酶脂质体微反应器(HLB)为均一圆球形,且平均粒径为5. 53±1. 75μm;以NaNO2作为模型,在浓度0. 05～10. 00 mmol/L范围内,所构建的生物传感器的峰电流密度与其浓度具有良好的线性关系,检出限为20. 8μmol/L (S/N=3),回归方程为Id=0. 83c+48. 68。

The objective of this study was to develop a biosensor composed of horseradish peroxidase( HRP) liposome bioreactor( HLB) and glassy carbon electrode( GCE) modified by chitosan( CS)using the self-assemble. HLB was successfully prepared and characterized,and then the optimization of temperature,pH value,scan rate and time were carried out. At last,the electrochemical properties of( HLB/CS)-GCE were evaluated by cyclic voltammetry( CV). The results showed that HLB had uniform spherical shape with a mean size of about 5. 53 ± 1. 75 μm. Fabricated biosensor showed a wider linear range of 0. 05-10. 00 mmol/L of sodium nitrite,and then the equation was Id= 0. 83 c +48. 68,with a detection limit of 20. 80 μmol/L estimated at a signal-to-noise ratio of 3.