一种荧光增强型硫化氢探针的设计合成及应用

Design,synthesis and application of a fluorescence enhanced hydrogen sulfide probe

通过3,4-二甲氧基苯胺与2-硝基苯甲醛一步反应,合成了一种荧光增强型的硫化氢探针S2,并通过核磁和质谱确定了S2的结构。紫外分光光度计测试表明,探针S2与硫化钠发生了反应。在PBS缓冲液中,10μmol/L探针S2与硫化钠反应后,通过荧光分光光度计进行荧光信号的检测。结果表明,探针S2可在8 min与硫化钠响应完全,随着硫化钠浓度的增加,探针S2在390 nm处的荧光值增加,且探针S2的荧光信号比值(F/F0)与硫化钠在0～8μmol/L的范围内呈线性关系,检出限为0. 20μmol/L。在与其他分析物的响应中,探针S2的选择性也很好。在自来水中通过加标回收实验,回收率在83. 3%～99. 4%之间。

A fluorescence enhanced hydrogen sulfide probe S2 was synthesized by the one-step reaction of 3,4-dimethoxyaniline and 2-nitrobenzaldehyde,and the structure of S2 was determined by NMR and MS. UV spectrophotometer showed that the probe S2 was reacted with sodium sulfide. In the PBS buffer,10 μmol/L probe S2 reacted with sodium sulfide and was detected by fluorescence spectrophotometer.The results showed that the probe S2 can respond completely at 8 min with sodium sulfide. With the increase of sodium sulfide concentration,the fluorescence value of the probe S2 at 390 nm increases,and the fluorescence signal ratio( F/F0) of the probe S2 was linearly related to the sodium sulfide in the range of 0 ~ 8 μmol/L,and the detection limit was 0. 20 μmol/L. The selectivity of probe S2 was also good in response to other analytes. The recovery rate was 83. 3% to 99. 4% in tap water.