液相色谱-串联质谱法检测猪肉中肾上腺素及其代谢物残留

Analysis of adrenaline drugs in pork by high performance liquid chromatography-tandem mass spectrometry

针对猪肉中新兴的肾上腺素及其代谢物3,4-二羟基扁桃酸、4-羟基-3-甲氧基-扁桃酸,基于"肾上腺素作用翻转"机理同步添加的异丙嗪,建立了超高效液相色谱-串联质谱检测方法.4种物质线性良好,相关系数均大于0.999;猪肉基质的定量限(LOQ, S/N=10)依次为25、100、5、0.1μg·kg^(-1),精密度(RSD)均小于10%,平均回收率在78.3%—92.5%.

A simultaneous multi-class analysis method for new adrenaline drugs and its metabolites (3,4-dihydroxymandelic acid and vanillylmandelic acid), and promethazine based on the mechanism of "epinephrine reversal" in pork by high performance liquid chromatography-tandem mass spectrometry was established.In the corresponding concentration range, 4 targets had good linear relationships with correlation coefficients more than 0.999. The limits of quantification (LOQs) were 25,100, 5, 0.1 μg·kg^-1 in pork, respectively. The average recoveries were 78.3%-92.5% and relative standard deviations were below 10%.