不同氮素基追比例对枸杞甜菜碱及相关酶含量的影响

Effects of Different Nitrogen Top Dressing Application Ratio on the Content of Betaine and Related Enzymes in Lycium barbarum L.

以5年生枸杞品种"宁杞7号"为试材,运用完全随机区组设计方法,设计1∶1、2∶1∶1、3∶1、3∶2∶1共4个不同氮肥追施比例水平,研究宁夏枸杞施氮不同追肥比例与甜菜碱含量及其代谢关键酶之间的关系。结果表明:氮肥不同追施比例对枸杞光合有明显的影响,前期追氮较少时光合速率相应较低,反之较高。整个生育期追肥比例以2∶1∶1时,其平均光合速率相对较高,说明枸杞生育期间氮肥相对均衡施用对其光合速率有益;氮肥的适度后移会相应增加枸杞单加氧酶含量,前期或后期追氮太多,均不利于枸杞单加氧酶累积;生育期内适度均匀施氮,利于枸杞甜菜碱醛脱氢酶(BADH)积累;甜菜碱含量与不同时期施氮量有直接关系,不同时期施氮较高时甜菜碱含量较高,667 m^2枸杞产量最高为276 kg(干质量)时氮肥追肥比例为2∶1∶1。

To study the relationship between the ratio of nitrogen fertilizer applied and the content of betaine and its metabolic key enzymes in Lycium barbarum L.four different top dressing nitrogen fertilizer applicationratio levels,namely 1∶1,2∶1∶1,3∶1 and 3∶2∶1 were adoptedby taking the five-year-old ’Ningqi No.7’ as the test material.The results showed that the ratio of top dressing nitrogen fertilizer application had a significant effect on the photosynthetic rate of Lycium barbarum L..The average photosynthetic rate was relatively high at 2∶1∶1 during the whole growth period,which indicated that the relatively balanced application of nitrogen fertilizer was beneficial to the photosynthetic rate.The content of monooxygenase would be increased in Lycium barbarum L.,while nitrogen fertilizer applicationdelayed,otherwise,too much nitrogen in the early or late growing stages.Application nitrogen evenly contributed to accumulation of betaine-aldehyde dehydrogenase(BADH) during growing season.The betaine content was directly related to the amount of nitrogen applied at different times,and the content was higher along with the nitrogen applied higher.The yield of Lycium barbarum L.was the highest at 2∶1∶1 with the ratio of nitrogen fertilizer applied,which was 276 kg per 667 m^2(dry weight).