苏打碱胁迫对玉米自交系苗期生长及生理特性的影响

Effect of Soda Alkali Stress on the Growth and Physiological Characteristics of Maize Inbred Lines at Seedling Stages

为进一步分析实验室前期获得的耐盐玉米自交系种质,本实验通过形态观察和生理指标测定来进行评价。结果表明,苏打碱胁迫7 d后,4个自交系株高均有所降低,只有郑58降低显著;郑58和WD郑58叶绿素含量显著提高,美-2叶绿素含量提高但不显著,黑玉米叶绿素含量略有降低但不显著;4个自交系丙二醛(MDA)含量均有所提高但都不显著,脯氨酸、可溶性糖含量和POD活性并没有显著上升。分析以上结果可能是实验胁迫强度或者胁迫时间不够,对这4个自交系细胞膜结构没有产生严重破坏,细胞没有产生过多的渗透调节物质以及保护酶来抵抗逆境胁迫。该实验结论可为以后玉米自交系盐碱胁迫鉴定提供参考。

In order to further analyze the salt-tolerant maize inbred lines, morphological observation and physiological detection were used in this study. The results showed that the height of 4 inbred lines decreased after 7 days of soda alkali stress, only‘Zheng 58’expressed significantly. Moreover, the chlorophyll content of‘Zheng 58’and‘WD Zheng 58’increased significantly, the chlorophyll content of‘Mei-2’increased but not significantly. In addition, the chlorophyll content of black maize decreased slightly but not significantly. Ultimately, the MDA content of the four inbred lines increased but not significantly;the content of proline and soluble sugar, the activity of POD did not increase significantly. The results implied that the stress intensity or stress time may be not enough to cause serious damage to the cell membrane structure, and the cell did not produce excessive osmotic regulators and protective enzymes to resist stress. The results can provide reference for identification maize inbred lines under soda alkali stress in the future.