马铃薯茎段高频再生体系的建立

Establishment of Stem-Based High-Frequency Regeneration Procedure for Potato

以大西洋、春薯3号、春薯5号、吉薯1号马铃薯脱毒试管苗为试验材料,对茎段再生体系进行研究,筛选与优化马铃薯再生体系的条件,以期建立马铃薯高频再生体系。结果表明:大西洋的愈伤组织诱导及分化效果最佳,诱导的愈伤组织呈鲜绿色,诱导出芽用时最短,诱导率和分化率分别为100%和95%。春薯3号、春薯5号、吉薯1号的分化率分别达79.4%、68.75%、85.7%。其中在添加2.0 mg/L 6-BA+0.5 mg/L 2,4-D+0.4 mg/L KT的MS培养基中,四个品种马铃薯的茎段均能形成愈伤组织,20 d愈伤组织诱导率均达100%。

The detoxification seedlings of potato cultivars‘Atlantic’,‘Chunshu 3’,‘Chunshu 5’and‘Jishu 1’were used as experimental materials, and the conditions of their stem tissue regeneration were explored and optimized with an aim to establish an efficient regeneration protocol in potato. The results showed that‘Atlantic’exhibited advantages over the 4 cultivars tested in terms of callus induction and differentiation. Under our experimental condition, Atlantic’s stem tissue sprout quickly and differentiated into callus of bright green with an induction rate and differentiation rate of 100% and 95%, respectively. The differentiation rates of other 3 cultivars were estimated to be 79.4% for‘Chunshu 3’, 68.75% for‘Chunshu 5’and 85.7% for‘Jishu 1’. Using the MS medium supplemented with 2.0 mg/L 6-BA + 0.5 mg/L 2, 4-D + 0.4 mg/L KT, the stem tissue of the 4 potato cultivars may be differentiated completely into callus within 20 days.