不同产地巫山淫羊藿指纹图谱的主成分、因子和聚类分析

Fingerprint of Epimedium wushanense Based on Principal Component,Factor and Cluster Analysis

目的:采用HPLC建立巫山淫羊藿的指纹图谱,结合主成分、因子和聚类分析对不同产地巫山淫羊藿综合品质进行研究,为其质量评价提供理论基础。方法:采用Agilent Infinity Lab Poroshe1l 120 SB-C18色谱柱(3. 0 mm×100 mm,2. 7μm),流动相乙腈(A)-水(B)梯度洗脱(0～5 min,25%～26%A; 5～6 min,26%～34%A; 6～11 min,34%～38. 5%A; 11～17 min,38. 5%～100%A; 17～20 min,100%A),流速0. 8 m L·min^(-1),检测波长270 nm,柱温30℃。结果:聚类分析较好地对不同产地的巫山淫羊藿进行分类,贵州产巫山淫羊藿和重庆巫山淫羊藿在聚类分析中明显分为二类,相距较远,说明由于环境气候的影响,不同产地的淫羊藿在品质上存在差异。主成分、因子分析结果,重庆巫山淫羊藿质量总体优于贵州产巫山淫羊藿,其中CQWS-02(重庆市巫山县官阳镇鸦鹊村),CQWS-10(重庆市巫山县官阳镇河湾)两个产地的样品在优质品种筛选时可重点考虑。另外,1号(朝藿定A),2号(朝藿定B),4号(淫羊藿苷),5号(未知成分)共有峰可作为巫山淫羊藿质量评价指标成分。结论:通过主成分、因子和聚类分析,可实现快速分析,发挥各自优势,互相验证和补充,多方面综合评判不同产地巫山淫羊藿药材的质量。

Objective:To establish the fingerprint of Epimedium wushanense by HPLC,and study the comprehensive quality of E.wushanense by combining principal components,factors and cluster analysis,so as to provide theoretical basis for its quality evaluation.Method:The chromatographic column was Agilent infinitylab poroshell 120 SB-C18(3.0 mm × 100 mm,2.7 μm),the flow phase was acetonitrile(A)-water(B)with a gradient of 0-5 min,25%-26% A;5-6 min,26%-34% A;6-11 min,34%-38.5% A;11-17 min,38.5%-100% A;17-20 min,100% A,the flow rate was 0.8 mL·min^-1,the detection wavelength was 270 nm,and the column temperature was 30 ℃.Result:The cluster analysis better classified E.wushanense from different producing areas.E.wushanense from Guizhou province and E.wushanense from Chongqing were classified as classⅡ.E.wushanense from Guizhou province and Chongqing were far apart,indicating that the quality of E.wushanense varies from place to place affected by environment and climate.The results of principal component analysis showed that the quality of E.wushanense produced in Chongqing was better than that of E.wushanense produced in Guizhou.Among them,CQWS-02(Yaque village,Guanyang town,Wushan county,Chongqing)and CQWS-10(Hewan,Guanyang town,Wushan county,Chongqing)can be considered in the selection of highquality varieties.In addition,No.1 common peak(epimedin A),No.2 common peak(epimedin B),No.4 common peak(icariin)and No.5 common peak(unknown component)in the fingerprint of the test samples could be used as the evaluation index components of E.wushanense quality.Conclusion:Principal components,factors and cluster analysis are used to achieve the rapid analysis,and their respective advantages are brought into full play for mutual verification and supplement.And the quality of E.wushanense in different origins can be comprehensively evaluated in all-round ways.