非标记蛋白定量方法在掺假肉鉴别中的应用

Application of non-labeled protein quantification method in the identification of adulterated meat

目的建立一种非标记蛋白定量法鉴别牛肉或羊肉中是否掺假。方法采用四极杆串联飞行时间高分辨质谱分析各种肉类蛋白酶解之后的样品,找到每种肉类特异的肽段,再用串联四极杆质谱测定特异肽段的定量结果,判定样本中是否有这种肉类存在。结果在测定的牛肉、羊肉、猪肉、鸡肉和鸭肉样本中,均找到每种肉类的特异肽段5～7条,从每种肉中选择3条肽段进行定量分析,可以很好地鉴别出牛羊肉中掺杂的其他肉类。结论该方法操作简便,结果可靠,灵敏度高,可用于在牛肉或羊肉中掺杂其他肉类的鉴别。

Objective To establish a non-labeled protein quantitative method for identification of adulteration in beef or mutton.Methods Quadrupole tandem time-of-flight high-resolution mass spectrometry was used to analyze the enzymatic hydrolyzed samples of various meat proteins and find the specific peptides of each meat.Quantitative results of the specific peptides were determined by quadrupole tandem mass spectrometry to identify whether the meat was present.Results In the samples of beef,mutton,pork,chicken and duck,5-7 specific peptides were found for each kind of meat.Three peptides were selected from each kind of meat for quantitative analysis,which could be very good to identify the beef and mutton adulterated with other meat.Conclusion The method is simple,reliable and sensitive,which can be used for the identification of beef or mutton adulterated with other meat.