高效液相色谱法测定婴幼儿配方乳粉中的维生素B_1、维生素B_2

Determination of vitamin B_1 and vitamin B_2 in infant milk powder by high performance liquid chromatography

目的建立高效液相色谱法快速测定婴幼儿配方乳粉中的维生素B1、B2的含量。方法样品进行前处理后,采用Waters Xbridge C18色谱柱(150 mm×4.6 mm, 5μm)进行分离。以0.05 mol/L乙酸钠溶液-甲醇为流动相等度洗脱,柱温为30℃,流速为1 mL/min,荧光检测器进行检测,外标法定量。结果在优化的实验条件下,维生素B1、维生素B2可以有效分离,线性范围为0.05～1.00μg/mL,相关系数均大于0.999。维生素B1的加标回收率为87.4%～99.1%,相对标准偏差为1.2%～3.06%,检出限为0.03 mg/100 g;维生素B2的加标回收率为90.6%～97.8%,相对标准偏差为0.97%～3.44%,检出限为0.02 mg/100 g。结论此方法简单、快捷、高效,适用于婴幼儿配方乳粉中维生素B1、维生素B2的测定。

Objective To establish a rapid method for the determination of vitamin B1 and B2 in infant milk powder by high performance liquid chromatography.Methods After the pretreatment,the samples were separated with Waters Xbridge C18 column(150 mm×4.6 mm,5 μm) by using 0.05 mol/L sodium acetate solution and methanol for isocratic elution at the flow of 1 mL/min.The temperature of column was 30 ℃.The samples were detected by fluorescence detector and quantified by external standard method.Results Vitamin B1 and vitamin B2 were separated efficiently under optimized experimental conditions.All of the linear range was 0.05-1.00 μg/mL and the correlation coefficients were higher than 0.999.The recoveries of spiked samples of vitamin B1 were 87.4%-99.1%,the relative standard deviations were 1.2%-3.06%,and the limit of detection was 0.03 mg/100 g.The recoveries of spiked samples of vitamin B2 were 90.6%-97.8%,the relative standard deviations were 0.97%-3.44%,and the limit of detection was 0.02 mg/100 g.Conclusion This method is simple,rapid and efficient,and it is suitable for the determination of vitamin B1 and vitamin B2 in infant formula milk powder.