高效液相色谱法测定保健食品原料和产品中角鲨烯的含量

Determination of squalene in health food and health food raw materials by high performance liquid chromatography

目的建立高效液相色谱法测定保健食品和保健食品原料中角鲨烯的含量。方法样品经乙腈提取,经过Waters Xbridge C18色谱柱分离以甲醇为流动相,流速为1.0 mL/min,柱温为40℃,在紫外检测器210 nm波长下测定角鲨烯的含量。结果当角鲨烯浓度在0.5～300μg/mL范围内线性良好(r=0.9999),方法检出限为0.719mg/kg,方法定量限为2.398mg/kg,相对标准偏差为3%,在复杂基质中方法平均回收率为92.4%,在单一基质中方法平均回收率为114.9%。使用市售样品和原料对该方法进行验证,检测值在商品标识值的97%～106%范围内。结论此方法操作便捷,适用性广,验证结果良好,适合对保健食品和保健食品原料中角鲨烯的含量检测。

Objective To establish a method for the determination of squalene in health food and health food raw materials by high performance liquid chromatography.Methods After extracting with acetonitrile,the samples were separated by Waters Xbridge C18 column using methanol as the mobile phase at the flow of 1.0 mL/min.The temperature of column was 40 ℃,and the squalene content was determined by ultraviolet detector at wavelength of 210 nm.Results When the squalene concentration varied from 0.5 to 300 μg/mL,the linearity was good(r=0.9999),the limit of detection of the method was 0.719 mg/kg,the limit of quantitation of the method was 2.398 mg/kg,and the relative standard deviation was 3%.The average recoveries were 92.4% in the complex matrix and 114.9% in the single matrix.The method was verified by commercial samples and raw materials,and the detected values were within the range of 97% to 106% of the commodity identification value.Conclusion This method is convenient to operate,has wide applicability and good verification results,which is suitable for the determination of squalene content in health food and health food raw materials.