基于F型mtDNA D-Loop的厚壳贻贝(Mytiluscoruscus)群体遗传多样性研究

STUDY ON THE GENETIC DIVERSITY OF MYTILUS CORUSCUS IN THE EAST CHINA SEA

为研究东海区厚壳贻贝(Mytilus coruscus)遗传多样性,以厚壳贻贝F mtDNA D-loop为标记,对浙江省舟山嵊山岛、宁波渔山岛、温州南麂岛、福建省宁德湾和莆田南日岛五个海区的厚壳贻贝群体进行了遗传分析。结果表明,厚壳贻贝各群体的遗传多样性差异不明显,在5个群体中,宁德群体的遗传多样性相对最丰富;将5个群体作为一个整体时,呈现出较高的单倍型多样性和较低的核苷酸多样性。对厚壳贻贝5个群体间的遗传分化系数(Fst)和基因流(Nm)进行检测,结果显示群体间Fst值都很低,但Nm值都很高(Nm绝对值>1),表明5个群体间存在丰富的基因交流。但宁德群体与浙江沿海的3个群体(嵊山、渔山、温州)的Fst值相对较高,且差异显著(P<0.5),表明宁德群体与这3个群体间出现遗传分化。本研究旨在为海洋经济贝类资源的保护管理提供理论依据。

To understand the genetic diversity of Mytilus coruscus in the East China Sea, the F mtDNA D-loop was used as a marker for M. coruscus populations sampled in five sea areas including Shengshi, Yushan, and Nanji Islands of Zhejiang Province, and Ningde Bay and Nanri Island of Fujian Province. Results show that the genetic diversity of these populations showed no significant difference. Among the five populations, the Ningde Bay population had the most abundant genetic diversity, and showed a higher haplotype and lower nucleotide diversity among the five populations. In addition, the genetic differentiation coefficient (Fst) and gene flow (Nm) among populations were determined. Among the five populations, that the Fst values were very low while the Nm values were high (Nm absolute value>1), indicating that there are abundant gene exchanges. However, the Fst values of Ningde population of Fujian Province and other three coastal populations of Zhejiang Province were relatively high, and the differences were significant (P<0.5), indicating that there was a genetic differentiation between the Ningde population and the three populations. The findings of the study may provide a theoretical basis for the protection and management of the marine economic shellfish resources.