摘要
目的:建立中药复方制剂茯黄颗粒的质量标准。方法:采用薄层色谱法(TLC)对处方中茯苓皮、黄芪、桑白皮、酒山萸肉进行鉴别,应用高效液相色谱法(HPLC)测定茯黄颗粒中马钱苷和黄芪甲苷的含量。结果:薄层色谱鉴别专属性强,分离度好;黄芪甲苷在3.295 05～32.950 5μg呈良好的线性关系,r=0.999 2,加样回收率为98.24%,RSD=1.94%;马钱苷在64.876 8～648.768μg呈良好的线性关系,r=0.999 5,加样回收率为99.93%,RSD=0.41%。结论:本方法准确可靠、灵敏度高,可用于茯黄颗粒的质量控制。
Objective:To establish quality standard of herbal compound prescription FuHuang granules.Methods:FuLingPi(Indian bread exodermis),HuangQi[Astragalus membranaceus(Fisch.)Bunge.],SangBaiPi(Morus alba L.)and JiuYuRou(common macrocarpium fruit)were identified using TLC,the contents of astragaloside A and loganin were measured using HPLC.Results:TLC showed strong identification and good resolution;astragaloside A presented good linear relationship in the range beween 3.295 05 and 32.950 5μg,r=0.999 2,sample recovery rate was 98.24%,RSD=1.94%;loganin showed good linear relationship in the range between 64.876 8 and 648.768μg,r=0.999 5,sample recovery rate was 99.93%,RSD=0.41%.Conclusion:The method,which is accurate,reliable and with high sensitivity,could be used for quality control of FuHuang granules.
作者
李富欣
于蓓蓓
刘瑾
孙丹丹
吴雪旖
闫雪生
LI Fuxin;YU Beibei;LIU Jin;SUN Dandan;WU Xueyi;YAN Xuesheng(Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong Academy of Chinese Medicine)
出处
《西部中医药》
2019年第2期44-48,共5页
Western Journal of Traditional Chinese Medicine
基金
山东省重点研发计划项目(编号2015GSF119029)
关键词
茯黄颗粒
质量标准
黄芪甲苷
马钱苷
FuHuang granules
quality standard
astragaloside A
loganin
