茯黄颗粒的质量标准研究

Study on Quality Standard of FuHuang Granules

目的:建立中药复方制剂茯黄颗粒的质量标准。方法:采用薄层色谱法(TLC)对处方中茯苓皮、黄芪、桑白皮、酒山萸肉进行鉴别,应用高效液相色谱法(HPLC)测定茯黄颗粒中马钱苷和黄芪甲苷的含量。结果:薄层色谱鉴别专属性强,分离度好;黄芪甲苷在3.295 05～32.950 5μg呈良好的线性关系,r=0.999 2,加样回收率为98.24%,RSD=1.94%;马钱苷在64.876 8～648.768μg呈良好的线性关系,r=0.999 5,加样回收率为99.93%,RSD=0.41%。结论:本方法准确可靠、灵敏度高,可用于茯黄颗粒的质量控制。

Objective:To establish quality standard of herbal compound prescription FuHuang granules.Methods:FuLingPi(Indian bread exodermis),HuangQi[Astragalus membranaceus(Fisch.)Bunge.],SangBaiPi(Morus alba L.)and JiuYuRou(common macrocarpium fruit)were identified using TLC,the contents of astragaloside A and loganin were measured using HPLC.Results:TLC showed strong identification and good resolution;astragaloside A presented good linear relationship in the range beween 3.295 05 and 32.950 5μg,r=0.999 2,sample recovery rate was 98.24%,RSD=1.94%;loganin showed good linear relationship in the range between 64.876 8 and 648.768μg,r=0.999 5,sample recovery rate was 99.93%,RSD=0.41%.Conclusion:The method,which is accurate,reliable and with high sensitivity,could be used for quality control of FuHuang granules.