水稻“9311”突变体库的氯酸盐毒性筛选与分析

Screening and Analysis for Rice “9311” Mutant Library by Chlorate

【目的】用氯酸盐筛选水稻(Oryza sativa)突变体库,分析氯酸盐对水稻的毒性。【方法】使用化学诱变剂甲基磺酸乙酯(Ethyl methanesulphonate,EMS)处理水稻材料"9311"种子,构建完成了水稻"9311"突变体库。【结果】通过水培法并利用氯酸盐的特性对诱变材料进行筛选,共筛选了2 361份诱变M2代材料,且与野生型相比获得了40份对氯酸盐表现差异敏感的材料。【结论】研究结果为进一步的材料创建与相关基因克隆提供了基础,也为水稻硝酸盐吸收与转化相关分子机理的研究奠定了基础。

[Purposes]To screen the mutant library of rice(Oryza sativa)was screened by chlorate and investigate the toxicity of chlorate to rice.[Methods]The chemical mutagen,ethyl methanesulphonate(EMS)was used to treat the seeds of rice material"9311”and construct the"9311"rice mutant library.[FindingslThe rice mutant library was screened by hydroponics and using the characteristics of chlorate.A total of 2361 mutant materials were screened and 40 lines showed a difference in sensitivity to chlorate compared with the wild type.In addition,the phenotypic differences of the 40 materials screened confirmed further candidate materials by further repeated experiments.[Conclusions]The results provided the basis for our further material creation and related gene cloning,and also laid the foundation for the study of molecular mechanisms related to nitrate uptake and transformation in rice.