某汽车制造厂工人柴油机尾气暴露对生物样本中8-OHdG水平影响

Effect of diesel exhaust exposure on 8-hydroxy-2' deoxyguanosine level in biological samples of automobile manufacturing workers

目的探讨血和尿中8-羟基脱氧鸟苷(8-OHdG)作为生物标志物评价柴油机尾气导致的人体DNA氧化损伤的可行性。方法采用方便抽样法,选择某汽车制造厂工作中接触柴油机尾气的56名男性工人为接触组,以不接触柴油机尾气的52名男性工人为对照组。采集2组工人8 h班后尿样和血样,以超高效液相色谱-质谱联用法检测尿和血浆中8-OHdG水平。结果接触组工人尿中8-OHdG水平高于对照组(中位数:2.54 vs 2.03μg/g肌酐,P<0.05);接触组工人血浆中8-OHdG水平与对照组工人比较,差异无统计学意义(中位数:32.20 vs 31.40 ng/L,P>0.05)。结论尿中8-OHdG可作为评价柴油机尾气接触所致人体氧化损伤的生物标志物。

Objective To investigate the feasibility of using 8-hydroxy-2’deoxyguanosine(8-OHdG) in blood and urine samples as biomarkers for the evaluation of human DNA oxidative damage caused by diesel exhaust(DE). Methods A convenient sampling method was used to select 56 male workers exposed to DE in a car manufacturing factory as exposure group, and 52 male workers without exposure to DE were selected as the control group.Urine samples and blood samples were collected from workers in the 2 groups 8 hours after work, and the levels of 8-OHdG in urine and plasma were measured by ultra-high performance liquid chromatography tandem mass spectrometer. Results The median level of urinary 8-OHdG in the exposure group was higher than that of control group(2.54 vs 2.03 μg/g Cr, P<0.05). The median levels of plasma 8-OHdG in the exposure group and control group showed no statistical significance(32.20 vs 31.40 ng/L, P>0.05). Conclusion The urinary 8-OHdG can be used as a biomarker for evaluating the oxidative damage induced by DE exposure.