NDRG2对唾液腺腺样囊性癌增殖、侵袭的影响

The effect of NDRG2 on proliferation and invasion of salivary adenoid cystic carcinoma

目的:探讨NDRG2基因在唾液腺腺样囊性癌组织中的表达及其对SACC-LM细胞增殖、侵袭的影响。方法:采用蛋白质免疫印迹法(Western blot)和实时荧光定量PCR(qRT-PCR)分析唾液腺腺样囊性癌组织及癌旁组织样本中NDRG2的表达,采用RNA干扰技术降低腺样囊性癌细胞SACC-LM中NDRG2基因的表达,利用CCK-8实验及集落形成实验检测细胞增殖能力的变化,利用划痕实验及Transwell实验分别检测细胞迁移及侵袭能力的变化,利用Western免疫印迹实验检测侵袭标志蛋白以及上皮-间充质转化(EMT)相关蛋白的表达。采用SPSS 17.0软件包进行数据处理。结果:NDRG2基因在唾液腺腺样囊性癌组织中的表达低于癌旁组织(P=0.017)。在降低NDRG2基因的表达后,SACC-LM细胞的增殖、迁移及侵袭能力显著增强(P<0.05),侵袭标志蛋白MMP2表达量显著升高(P=0.0004),上皮标志物E-Caherin表达量显著降低(P=0.0229),间充质标志物N-Cadherin表达量显著升高(P=0.0009)。结论:NDRG2基因在腺样囊性癌中低表达,降低NDRG2的表达可增强SACC-LM的增殖、迁移及侵袭能力。

PURPOSE: To investigate the expression of NDRG2 gene and its role in the progress of salivary adenoid cystic carcinoma(SACC). METHODS: Western blot assay was used to analyze the expression level of protein of SACC.The mRNA level was analyzed by qRT-PCR assay. The expression level of NDRG2 in SACC-LM was downregulated by small interfering RNA. The proliferation of SACC-LM was detected by CCK-8 assay and colony formation assay. Wound healing assay and Transwell invasion assay were performed to observe the abilities of migration and invasion, respectively.The expression of invasion markers and EMT-related proteins was examined by Western blot. SPSS 17.0 software package was used for statistical analysis. RESULTS: NDRG2 expression was decreased in SACC tissues(P =0.017). Down-regulation of NDRG2 promoted proliferation,migration and invasion of SACC-LM(P<0.05). The expression of MMP2(P=0.0004) was up-regulated. The epithelial marker E-Cadherin was down-regulated(P =0.0229) while the mesenchymal marker N-Cadherin was increased(P=0.0009). CONCLUSIONS: NDRG2 is decreased in SACC and Si-NDRG2 could enhance proliferation,migration and invasion of salivary adenoid cystic carcinoma cell line SACC-LM.