紫草标准汤剂成分分析及其抗银屑病作用研究

Components analysis and anti-psoriatic effect of standard decoction of Lithospermum

目的分析紫草标准汤剂中主要成分,观察其抗银屑病作用。方法采用浸出物测定法测定紫草标准汤剂出膏率,采用分光光度法测定紫草标准汤剂总多糖、总酚酸含量;观察不同浓度紫草标准汤剂对咪喹莫特诱导银屑病样小鼠皮损的干预作用。结果紫草标准汤剂出膏率为4.65%,总多糖含量为1.182mg/ml,总酚酸含量为3.506mg/ml;不同浓度紫草标准汤剂可改善银屑病样小鼠皮肤出现的鳞屑、红斑、浸润症状,减少表皮厚度。结论紫草标准汤剂可显著改善咪喹莫特诱导的小鼠银屑病样皮损,具有抗银屑病作用。

Objective To analyze the main ingredients and investigate the anti-psoriasis effect in the standard decoction of Lithospermum. Methods The extraction rate was determined by extract determination method, and the content of total polysaccharide and total phenolic acid was evaluated by spectrophotometry. Moreover, the effects of different concentrations of the standard decoction of Lithospermum on skin lesions induced by imiquimod (IMQ) in psoriatic mice were observed. Results The extracting rate was 4.65 %, the total polysaccharide content was 1.182 mg/ml and the total phenolic acid content was 3.506 mg/ml. The different concentrations of the standard decoction of Lithospermum could ameliorate the scales, erythema and psoriasis-like mice skin and reduce the thickness of epidermis. Conclusions The standard decoction of Lithospermum could improve the psoriasis-like lesions induced by imiquimod in mice and possess the anti-psoriasis effect.