雌激素受体β基因多态性与绝经后女性原发性良性阵发性位置性眩晕的相关性分析

Association of estrogen receptor β gene polymorphism and primary benign paroxysmal positional vertigo in postmenopausal women

目的探讨雌激素受体(ER)β基因多态性与绝经后女性原发性良性阵发性位置性眩晕(BPPV)的关联性。方法选取2015年1月至2017年7月于本科治疗的165例绝经后女性原发性BPPV患者作为病例组,以及同期行健康体检的160例正常绝经的健康女性作为对照组,采用聚合酶链反应-DNA直接测序法检测ERβ基因G1082A、G1730A和CA重复序列多态性,并收集患者临床资料进行分组及统计学分析。结果病例组与对照组比较,两组ERβ基因G1082A、G1730A位点基因型和等位基因分布频率差异均无统计学意义(均P>0.05);两组CA重复序列多态性位点基因型分布频率差异具有统计学意义(P<0.05),携带短CA重复序列可显著增加绝经后女性原发性BPPV发病风险(OR=1.446,95%CI 1.061～1.969,P=0.019)。结论 ERβ基因CA重复序列多态性可能与绝经后女性原发性BPPV易感性相关,短CA重复序列可能是绝经后女性原发性BPPV的易感基因;ERβ基因G1082A、G1730A多态性与绝经后女性原发性BPPV易感性均无明确关联。

Objective To investigate the genetic association between estrogen receptor(ER)βgene polymorphism and primary benign paroxysmal positional vertigo(BPPV)in postmenopausal women.Method 165 ostmenopausal women with primary BPPV from January 2015 to July 2017 were recruited in this study as the case group.Meanwhile,160 normal menopausal women were included as control.The genotype was determined by polymerase chain reaction-DNA direct sequencing analysis for the G1082A,G1730A and CA dinucleotide repeat polymorphism of ERβgene.To collect other clinical data for statistical analysis.Results Genotype and allele frequencies of G1082A and G1730A of ERβgene did not differ significantly between two groups(all P>0.05).There were significant difference in the genotype frequencies of CA repeats of the ERβgene between two groups(χ^2=6.914,P=0.032).The risk of BPPV was increased for ostmenopausal women with short CA repeats compare to them with long repeats(OR=1.446,95%CI 1.061-1.969,P=0.019).Conclusion The polymorphisms of CA repeats of the ERβgene are correlated with the susceptibility of primary BPPV for postmenopausal women,in which short CA repeats allele may be associated with the genetic susceptibility to BPPV.The distributions of three genotype of G1082A and G1730A of ERβgene in the two groups have no obvious differences,suggesting it has no evident correlation with the onset of BPPV.