Fonsecaea monophora色素对人巨噬细胞炎症细胞因子表达的影响

Effect of Fonsecaea monophora pigment on the expression of inflammatory cytokines in THP-1-derived human macrophages

目的探究Fonsecaea monophora(F. monophora)色素对THP-1来源的人巨噬细胞炎症细胞因子表达的影响。方法将THP-1人单核细胞诱导为巨噬细胞,分别与F. monophora的标准株、色素株和白化株进行共培养,细胞免疫荧光检测细胞膜表面分子CD14和CD68的表达;定量PCR检测各组中IL-1β、TNF-α、IL-6、IL-10和TGF-β的表达。结果 THP-1人单核细胞诱导为巨噬细胞后,膜表面分子表达由CD14转变为CD68。与F. monophora共培养后,标准株上调IL-6、IL-10和TGF-β的表达(P均<0.05),色素株上调IL-6和TGF-β的表达(P均<0.05),白化株上调IL-1β、TNF-α、IL-6和IL-10的表达(P均<0.05);白化株较标准株能上调IL-1β的表达(P <0.05),标准株较色素株和白化株能上调IL-10和TGF-β的表达(P均<0.05)。结论 F. monophora标准株能促使人巨噬细胞往抗炎状态发展,白化株能促使人巨噬细胞往促炎状态发展,色素株则使人巨噬细胞处于促炎和抗炎的平衡状态,F. monophora色素可能会导致宿主炎症状态紊乱。

Objective To evaluate the effect of Fonsecaea monophora (F. monophora) pigment on the expression of inflammatory cytokines in THP-1-derived human macrophages. Methods THP-1 human monocytes were induced into macrophages and co-cultured with the standard, pigment and albino strains of F. monophora. The expression levels of CD14 and CD68 on the cell membrane surface were quantitatively detected by immunofluorescence. Real-time quantitative PCR was performed to detect the expression o fIL-1β, TNF-α, IL-6, IL-10 and TGF-β in each group. Results After the induction of THP-1 human mononuclear cells into macrophages, the expressed membrane surface molecule was changed from CD14t o CD68. After coculture with F. monophora, the expression levels of IL-6, IL-10 and TGF-β were significantly up-regulated in the standard strain( all P < 0.05). The expression of IL-6 and TGF-β was remarkably up-regulated in the pigment strain( both P < 0.05). The expression levels of IL-1β, TNF-α, IL-6 and IL-10 were significantly up-regulated in the albino strai(n all P< 0 .05). The expression of I-L1β in the albino strain was significantl y higher than that in the standard strai(n P< 0 .05). The expression levels of I-L10 and TGF-β were significantly up-regulated in the standard strain compared with those in the pimgent and albino strains( all P < 0.05). Conclusions The standard strain of F.monophora can promote the development of human macrophages into the anti-inflammatory state. The albino strain can accelerate the progression of human macrophages into the proinflammatory state, whereas the pigment strain can maintain human macrophages in het balance state between pro-inflammatory and anti-inflammatory state, indicating that F. monophora pigment may lead to the disorder of inflammatory state of the host.