碱蓬SgP5CS基因过表达提高拟南芥耐旱性

Overexpression of SgP5CS gene from Suaeda salsa enhances drought tolerance of transgenic Arabidopsis

脯氨酸是植物中的渗透调节物质,Δ~1-吡咯啉-5-羧酸合成酶(P5CS)是其合成过程中重要的调控因子。为探究P5CS基因功能,克隆了碱蓬SgP5CS基因并导入拟南芥,使其在拟南芥中过表达,然后进行相关指标测定和耐旱性鉴定。结果显示,干旱胁迫诱导2周后,SgP5CS过表达的拟南芥植株具有较长的根系,同时游离脯氨酸的含量显著增加,丙二醛含量显著降低,表明SgP5CS基因在拟南芥中过表达能够增强拟南芥耐旱性。研究结果为深入了解碱蓬中SgP5CS基因的功能及其在植物中的抗旱机制奠定了基础。

Δ^1-pyrroline-5-carboxylate synthetase(P5 CS) is an important regulatory factor in synthesis of proline, an osmotic regulator in plants. SgP5CS gene of Suaeda salsa was cloned and introduced into Arabidopsis, the related physiological indexes and drought tolerance were determined, and the function of SgP5CS gene and regulation mechanism under drought stress were studied. The results showed that Arabidopsis with SgP5CS overexpression had long roots, while the content of free proline increased significantly and content of malondialdehyde decreased after two weeks of drought stress. It was found that overexpression of SgP5CS gene could enhance drought tolerance of transgenic Arabidopsis. These results laid a foundation to further study the SgP5CS gene function in Suaeda glauca, which might provide reference for the drought resistance mechanism in plants.