NaCl胁迫对玉米种子萌发特性及α-淀粉酶活性的影响

Effects of NaCl Stress on Seeds Germination Characteristic and α-amylase Activity of Maize Seed

为研究玉米的耐盐机理以金帅2008、玉帅2008、河北邯郸585、晋中黑玉米种子为试验材料,分别用0、30、90、120、150、180 mmol·L^(-1 )NaCl溶液处理,对玉米种子的发芽率、发芽势、发芽指数及其萌发过程中α-淀粉酶的活性进行测定。结果表明:金帅2008、玉帅2008在NaCl浓度低于60 mmol·L^(-1)、河北邯郸585在NaCl浓度低于30 mmol·L^(-1)时,种子的萌发指标及α-淀粉酶活性有一定升高,超过此浓度时萌发指标及α-淀粉酶活性又逐渐降低;晋中黑玉米种子的萌发指标及α-淀粉酶活性随着NaCl浓度的升高,而呈现下降趋势。

In order to study the salt tolerance mechanism of maize,the seeds of Jinshuai 2008,Yushuai 2008,Hebei Handan 585 and Jinzhong black maize were treated with 0,30,90,120,150 and 180 mmol ·L^-1 NaCl solution respectively.The germination rate,germination potential,germination index and the activity of alpha-amylase in the process of maize seed germination were determined.The results showed that when NaCl concentration was lower than 60 mmol ·L -1 in Jinshuai 2008 and Yushuai 2008,and when NaCl concentration was lower than 30 mmol ·L^-1 in Hebei Handan 585 the germination index and alpha-amylase activity of seeds increased to a certain extent,but when the above concentration was higher,the germination index and alpha-amylase activity of seeds decreased gradually;and the germination index and alpha-amylase activity of seeds of Jinzhong black maize decreased with increase of NaCl concentration.