烟草PPO氨基酸序列分析

Analysis of Amino Acid Sequence of Tobacco PPO

为研究烟草PPO的理化特性,采用PROTPARAM、SMART和NPSA-PRABI工具解析烟草PPO成员的生理特性、结构域和二级结构。结果表明:烟草PPO为酸性氨基酸,NtPPO1、NtPPO2和NtPPO3属于不稳定的蛋白,其中以NtPPO1的耐热性相对较强,三者共同含量较高的氨基酸为亮氨酸和脯氨酸;NtPPO1、NtPPO2和NtPPO3均含有Tyrosinase、PPO1_DWL和PPO1_KFDV结构域,且NtPPO1和NtPPO2含有信号肽;烟草PPO成员的二级结构主要为无规则卷曲,其次是α-螺旋,各二级结构排列较为分散。

In order to study the physicochemical properties of tobacco PPO,the physiological characteristics,domain and secondary structure of tobacco PPO members were analyzed by PROTPARAM,SMART and NPSA-PRABI tools.The results showed that tobacco PPO was acidic amino acid,NtPPO1,NtPPO2 and NtPPO3 belonged to unstable protein,among which NtPPO1 had relatively strong heat resistance,and the three amino acids with higher common content were leucine and valine;NtPPO1 both NtPPO2 and NtPPO3 contain Tyrosinase,PPO1_DWL and PPO1_KFDV domains,and NtPPO1 and NtPPO2 contain signal peptides;the secondary structure of tobacco PPO members was mainly irregular coiling,followed by α-helix,and the secondary structure was more dispersed.