一种自制药酒功效及挥发性成分分析

Efficacy and Volatile Component Analysis of a Self-made Medcine Wine

自制药酒的保健功能通常被长期饮用者所验证,但是其功能成分却并不清楚,本实验研究了一种颇受当地群众喜爱的药酒的功效及风味成分.经硝酸铝比色法测定酒中黄酮含量,香草醛-高氯酸法测定皂苷含量,DNS法测定多糖、总糖含量,考马斯亮蓝-G250法测定蛋白质含量,紫外分光法测定游离氨基酸含量,原子荧光光谱法测定硒含量,原子吸收光谱法测定锌、铜含量,结果显示:自制药酒中黄酮含量为84.12 mg/100 mL,RSD%=1.61%;皂苷含量为56.49 mg/100mL,RSD%=0.59;粗多糖含量为54.45mg/100mL,RSD%=0.18;总糖含量为760.60mg/100mL、RSD%=0.38;蛋白质含量为48.12 mg/100 mL,RSD%=0.06;游离氨基酸为11.90 mg/100 mL,RSD%=0.01;铜含量为1.88 mg/100 mL,RSD%=0.004;锌和硒均未检出.此外,经GC-MS测定,酒中含有27种风味成分,主要包括酯类、醇类两大类,其中酯类12种,醇类6种,己酸乙酯和庚酸乙酯为自制药酒的主体风味物质,另外还有少量的烷烃和酸类物质.结果表明:自制药酒中富含多糖、黄酮、皂苷、氨基酸、蛋白质等活性成分.

The health function of homemade medicinal liquor is usually validated by long-term drinkers,but its functional components are not clear.This experiment studied the efficacy and flavor components of a kind of medicinal liquor which is popular with local people.Flavone content in wine was determined by aluminium nitrate colorimetry,saponin content by vanillin-perchloric acid method,polysaccharide and total sugar content by DNS method,protein content by Coomassie brilliant blue-G250 method,free amino acid content by ultraviolet spectrophotometry,selenium content by atomic fluorescence spectrometry,zinc and copper content by atomic absorption spectrometry.The results showed that the content of flavone was 84.12 mg/100 mL,RSD%=1.61%;saponin was 56.49 mg/100 mL,RSD%=0.59;crude polysaccharide was 54.45 mg/100 mL,RSD%=0.18;total sugar content was 760.60 mg/100 mL,RSD%=0.38;protein content was 48.12 mg/100 mL,RSD%=0.06;free amino acid was 11.90 mg/100 mL,RSD%=0.01;copper content was 1.88 mg/100 mL,RSD%=0.004;zinc and selenium were not detected.In addition,by GC-MS analysis,the wine contains 27 flavor components,including esters and alcohols,including 12 esters,6 alcohols,ethyl caproate and ethyl heptanoate as the main flavor substances of self-made wine,and a small amount of alkanes and acids.The results showed that the self-made medicinal liquor was rich in polysaccharides,flavonoids,saponins,amino acids,proteins and other active ingredients.