蛇六谷提取物抑制K562细胞增殖并诱导分化的作用

Effect of TuAKe on proliferation and differentiation of K562 cells

目的:探讨蛇六谷提取物(TuAKe)抑制人慢性髓系白血病K562细胞增殖和诱导分化的作用机制。方法:不同浓度的TuAKe处理K562细胞,MTT比色法和半固体集落形成实验检测K562细胞的增殖能力;瑞氏-姬姆萨染色观察细胞形态;流式细胞术检测分化相关抗原CD11b、CD14和CD42b的阳性表达率;Western blot法检测细胞周期蛋白依赖性激酶2(CDK2)、细胞周期蛋白E1(cyclin E1)和红系分化核转录因子GATA-1的蛋白表达水平。结果:TuAKe能够抑制K562细胞增殖,改变细胞形态,抑制K562细胞进入S期,并阻滞在G_2/M期,提高分化相关抗原CD11b、CD14和CD42b阳性表达率,上调GATA-1蛋白表达,同时下调CDK2和cyclin E1蛋白表达(P<0.05)。结论:TuAKe可能通过调控细胞周期抑制K562细胞增殖,同时诱导K562细胞多向分化。

AIM: To investigate the effects of Sheliugu extract(the extract from Amorphophallus konjac tuber, TuAKe) on the proliferation and differentiation of human chronic myeloid leukemia K562 cells. METHODS: TuAKe at different concentrations was used to treat with the K562 cells. The colony formation ability of the cells was detected by the colony formation assay with semi-solid culture. The viability of K562 cells was measured by MTT assay. The morphological changes of the cells were observed under microscope with Wright-Giemsa staining. The positive expression rates of differentiation-related antigens CD11 b, CD14 and CD42 b were analyzed by flow cytometry. The protein expression of cyclin-dependent kinase 2(CDK2), cyclin E1 and erythroid cell differentiation nuclear transcription factor GATA-1 was determined by Western blot. RESULTS: TuAKe reduced the proliferation of K562 cells, reduced nuclear mass ratio, inhibited K562 cells to enter S phase, and blocked the cells in G2/M phase. TuAKe improved the positive expression rates of differentiation-related antigens CD11 b, CD14 and CD42 b, increased GATA-1 protein expression, and decreased the protein expression of CDK2 and cyclin E1(P<0.05). CONCLUSION: TuAKe may inhibit K562 cell proliferation by regulating cell cycle, and induce K562 cell multidirectional differentiation at the same time.