雌激素受体拮抗剂对泌乳素腺瘤GH_3细胞增殖的影响

Effects of Estrogen Receptor Antagonists on the Growth and Proliferation of Pituitary GH_3 Cell

目的:探讨雌激素受体拮抗剂对泌乳素腺瘤细胞增殖的影响。方法:在同样去激素培养的条件下添加不同浓度雌激素和雌激素受体拮抗剂,应用MTT法检测其对GH_3细胞增殖的影响。结果:不同浓度的OHTam对GH_3细胞有明显的抑制作用,并且与剂量呈正相关,10^(-11) mol/L即有抑制作用,10^(-6) mol/L抑制作用达到最大。E_2可显著的刺激GH_3细胞增殖,10^(-12)mol/L即有促进增殖作用,10^(-9) mol/L作用达到最大,而后随着浓度的升高增殖作用反而逐渐减弱。结论:雌激素受体拮抗剂能显著抑制泌乳素腺瘤细胞的生长,理论上可以用于泌乳素腺瘤的药物治疗。

Objective:To investigate the effect of estrogen receptor antagonists on the in vitro growth of human prolactinomas.Method:Estradiol and estrogen receptor antagonists were added respectively at different concentrations into the culture medium MTT assays were used to detect the effects on the growth and proliferation of GH3 cells.Result:OHTam could inhibit GH3 cell proliferation as dose-dependent manner,when the concentration was 10^-11 mol/L,the GH3 cell proliferation was inhibit.If the concentration rises to 10^-6 mol/L,it would produce the greatest effect.E2 had significant stimulatory effect on growth of GH3 cells,and the effect worked even at low concentration of 10^-12 mol/L.The greatest effect would produce when the concentration was 10-9 mol/L,if the concentration still increases,the proliferation effect would gradually weaken.Conclusion:Estrogen receptor antagonists can inhibit GH3 cell proliferation.In theory,it can be used for the drug treatment of prolactinomas.