云南产卷烟中游离氨基酸含量分析

Content analysis for free amino acids in cigarettes produced in Yunnan

为明确云南产卷烟中游离氨基酸的含量特征,分析了40仹云南产卷烟样品中的19种游离氨基酸含量。结果表明:云南产卷烟的脯氨酸含量最高,达2 270.55μg/g,天冬氨酸含量为220.98μg/g,苯丙氨酸含量为144.03μg/g;谷氨酰胺、缬氨酸、色氨酸、组氨酸、谷氨酸、酪氨酸、胱氨酸、丙氨酸、赖氨酸、精氨酸、苏氨酸和丝氨酸含量为10.59～81.37μg/g,异亮氨酸、亮氨酸、天冬酰胺和甘氨酸含量均低于10μg/g。除赖氨酸与其他氨基酸不相关外,其余氨基酸之间均存在中等程度或高度相关性。对卷烟品质贡献较大的有酪氨酸、组氨酸、丝氨酸、谷氨酰胺、苏氨酸、色氨酸、苯丙氨酸、精氨酸、亮氨酸、异亮氨酸、天冬酰胺、缬氨酸和脯氨酸。40仹卷烟样品可聚为3类,聚类结果与样品实际品质较为吻合。

To investigate the amino acid content of cigarette produced in Yunnan, the contents of 19 kinds of free amino acids in 40 samples of cigarettes produced in Yunnan were analyzed. The results showed that the mean content of proline is the highest (2 270.55 μg/g), followed by aspartic acid (220.98 μg/g) and phenylalanine (144.03 μg/g). The contents of glutamine, valine, tryptophan, histidine, glutamic acid, tyrosine, cystine, alanine, lysine, arginine, threonine and serine ranged from 10.59 to 81.37 μg/g, and those of isoleucine, leucine, asparagine and glycine were lower than 10 μg/g. There were moderate or high correlations among amino acids except for lysine. Tyrosine, histidine, serine, glutamine, threonine, tryptophan, phenylalanine, arginine, leucine, isoleucine, asparagine, valine and proline contributed significantly to cigarette quality. The 40 cigarette samples could be clustered into 3 types, and the clustering results agreed well with the actual quality of the samples.