内蒙古呼和浩特地区鸡源沙门氏菌分离鉴定、血清学鉴定及耐药性研究

Isolation, identification, serotypic characterization and antimicrobial resistance analysis of recently isolates of Salmonella from chickens in Hohhot of Inner Mongolia

目的 了解内蒙古呼和浩特地区市售整鸡的沙门氏菌污染状况,为建立食物污染及公共卫生安全提供数据支持。方法 2011～2012年仍呼和浩特地区采集冷冻、冷藏和现宰杀整鸡样品,迚行沙门氏菌的定性检测、血清分型及耐药性研究。结果 不同状态整鸡样品的沙门氏菌携带率明显不同,冷藏整鸡沙门氏菌检出率显著高于冷冻和现宰杀整鸡。血清型主要为肠炎沙门氏菌(Salmonella enteritidis)。36株沙门氏菌均对头孢替坦、亚胺培南最为敏感,对氨苄西林、哌拉西林、呋喃妥因、氨苄西林/舒巴坦、头孢唑林等表现出不同程度的耐药性,多重耐药菌株中五耐最为严重(25%)。结论 内蒙古呼和浩特地区鸡肉中的沙门氏菌多重耐药现象比较严重。

Objective To understand the situation of Salmonella contamination in raw chicken in Hohhot in Inner Mongolia, in order to provide basic data support for the establishment of the precaution system on food poisoning and public health security. Methods The raw chicken samples of frozen, refrigerated and fresh butchered were collected from 2011 to 2012 in Hohhot. The samples were tested for Salmonella contamination, serotypes and drug resistance. Results The Salmonella carrier rate of whole chicken samples in different states was significantly different. The detection rate of Salmonella in frozen whole chickens was significantly higher than that of frozen and slaughtered whole chickens. The serotype was mainly Salmonella enteritidis. Thirty-six strains of Salmonella were most sensitive to cefotetan and imipenem, and showed different degrees of resistance to Ampicillin, Piperacil lin,Nitrofurantoin, Ampicillin/Sulbactam, Cefazolin, and multi-drug resistant strains. The five-resistant was the most serious(25%). Conclusion Salmonella isolates recovered from chicken in Hohhot of Inner Mongolia are seriously resistant to antimicrobial commonly used as human and veterinary medicine.