摘要
目的:探讨精子线粒体DNA(mtDNA)4 977bp片段缺失与男性不育的相关性。方法:采用聚合酶链反应(PCR)技术对200例标本进行了精子mtDNA 4 977bp片段缺失的研究,其中精子活力正常标本(对照组)和弱精子症患者(观察组)各100例。结果:弱精子症患者中精子mtDNA 4 977bp片段缺失率为64%,正常对照组mtDNA 4 977bp片段缺失率为6%,两组差异具有明显统计学意义(P<0.01)。结论:精子mtDNA 4 977bp片段缺失与弱精症以及男性不育方面有一定的相关性。
Objective:To investigate the relationship between the deletion of 4 977bp fragment of sperm mitochondrial DNA(mtDNA)and male infertility.Methods:Polymerase chain reaction(PCR)was used to study the deletion of sperm mtDNA 4 977 bp fragment in 200 specimens,including 100 cases of normal sperm motility(control group)and 100 cases of asthenospermia(observation group).Results:The deletion rate of sperm mtDNA 4 977bp fragment was 64%in asthenospermia patients and 6%in normal control group.There was significant difference between the two groups(P<0.01).Conclusion:The deletion of sperm mtDNA 4 977bp fragment is correlated with asthenospermia and male infertility.
作者
盛礼建
王巧刚
王巧燕
SHENG Lijian;WANG Qiaogang;WANG Qiaoyan(Dongyang People’s Hospital of Zhejiang Provice 322100)
出处
《医学理论与实践》
2019年第7期946-947,958,共3页
The Journal of Medical Theory and Practice
基金
浙江省医药卫生科技计划(2014KYA220)
关键词
男性不育
聚合酶链式反应
线粒体DNA
缺失
Male infertility
Polymerase chain reaction
Mitochondrial DNA
Deletion
