自然流产患者的卵泡液代谢标志物研究

Study of follicular fluid metabolic markers in patients with spontaneous abortion based on follicular fluid metabonomics

目的探讨与自然流产发生相关的卵泡液(FF)代谢标志物,以进一步科学阐释自然流产的发生机制。方法利用超高效液相色谱-高分辨质谱技术对因输卵管因素行体外受精-胚胎移植(IVF-ET)后自然流产患者即流产组(40例)及分娩者即对照组(45例)的FF进行代谢组学分析。结果流产组和对照组FF共32种差异代谢物,主要有氨基酸类、胆固醇类、维生素类、脂肪酸类、胆酸类、溶血卵磷脂类及其他7大类,其中上调的有Dehydroepiandrosterone sulfate,Lyso PC(16∶0),Palmitoylglycerophosphocholine等8种,下调的有L-valine,6-Oxohexanoate,L-Phenylalanine,2-Oxoglutarate,Linoleate等24种。结论本研究鉴定的32种差异代谢物与自然流产相关,为自然流产提供可能的诊断依据和治疗靶点,从FF代谢组学层面为阐释自然流产发生机制提供科学依据。

Objective To investigate the follicular fluid metabolic markers associated with spontaneous abortion to further elucidate the mechanism of spontaneous abortion.Methods Follicular fluid metabonomics analysis was performed on abortion group(n=40)and control group(n=45)who underwent in vitro fertilization-embryo transfer(IVF-ET)by ultra-high performance liquid chromatography-high resolution mass spectrometry.Results There were 32 kinds of different follicular fluid metabolites between abortion group and control group,including amino acids,cholesterol,vitamins,fatty acids,bile acids,lysolecithin and others.Dehydroepiandrosterone sulfate,LysoPC(16:0),Palmitoylglycerophosphocholine and other 5 metabolites were up-regulated,and 24 species were down-regulated,including L-valine,6-Oxohexanoate,L-Phenylalanine,2-Oxoglutarate,Linoleate and so on.Conclusions The 32 differential metabolites identified in this study are related to spontaneous abortion and provide a possible diagnostic basis and therapeutic target for spontaneous abortion.It provides a scientific basis for elucidating the mechanism of spontaneous abortion from the perspective of follicular fluid metabonomics.